[CAS NO. 1002789-86-7] TZ9
PRODUCTS SPECIFICATIONS [1002789-86-7]
DESCRIPTION [1002789-86-7]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 366.33 |
| Molecular Formula | C17H14N6O4 |
| SMILES | O=C(OCC1=NC(N)=NC(NC2=CC=CC=C2)=N1)C3=CC=C([N+]([O-])=O)C=C3 |
1 Publications Citing Use of MCE
Summary
In Vitro
TZ9 (Rad6B SMI #9) (0.5-100 µM; 72 h) inhibits MDA-MB-231 cell proliferation and migration
[1]
.
TZ9 (0.1-5 µM; 24-72 h) delays cell-cycle progression in MDA-MB-231 cells
[1]
.
TZ9 (5 µM; 8-48 h) induces apoptosis in MDA-MB-231 cells
[1]
.
TZ9 (0.5, 1, 2.5, 5 µM; 24 h) inhibits H2A ubiquitination and downregulates levels of PCNA and β-catenin protein in MDA-MB-231 cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [1]
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0.5-100 µM |
| Incubation Time: | 72 h |
| Result: | Inhibited MDA-MB-231 cell proliferation with IC 50 ~6µM. |
Apoptosis Analysis [1]
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 5 µM |
| Incubation Time: | 8-48 h |
| Result: | Induced cells apoptosis. |
Cell Cycle Analysis [1]
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0.1-5 µM |
| Incubation Time: | 24-72 h |
| Result: |
Increased the proportion of G2-M-arrested cells by 2-fold and was accompanied by a proportional decrease in S-phase cells when at 24 h.
Significantly increased the percentage of cells with cytoplasmic/nuclear cyclin B1 staining. |
Western Blot Analysis [1]
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0.5, 1, 2.5, 5 µM |
| Incubation Time: | 24 h |
| Result: | Inhibited H2A ubiquitination, decreased PCNA and β-catenin protein levels. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 40 mg/mL ( 109.19 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.7298 mL | 13.6489 mL | 27.2978 mL |
| 5 mM | 0.5460 mL | 2.7298 mL | 5.4596 mL |
| 10 mM | 0.2730 mL | 1.3649 mL | 2.7298 mL |
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1.
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2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.08 mg/mL (5.68 mM); Clear solution