[CAS NO. 1033781-20-2] KS15

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PRODUCTS SPECIFICATIONS [1033781-20-2]

Catalog

HY-115672

Brand

MCE

CAS

1033781-20-2

DESCRIPTION [1033781-20-2]

Overview

MDL	-
Molecular Weight	420.30
Molecular Formula	C20H22BrNO4
SMILES	CCO[C@@H](C(O)=O)CC1=CC(/C(C)=N/OCC2=CC=C(C=C2)Br)=CC=C1

For research use only. We do not sell to patients.

Summary

KS15 is an inhibitor of the interactions between cryptochromes (CRYs: CRY1 and CRY2) and the CLOCK:BMAL1 heterodimer . KS15 impairs the feedback actions of CRYs on E-box-dependent transcription (EC ₅₀ =4.9 μM) by CLOCK:BMAL1 heterodimer, an indispensable transcriptional regulator of the mammalian circadian clock. Anti-proliferative activity ^[1] ^[2] .

In Vitro

Applications of KS15 at different concentrations from 1 μM to 10 μM restored the E-box-driven luciferase activities in a dose-dependent manner, indicating that KS15 evidently impairs the suppressive actions of CRYs on CLOCK:BMAL1-induced transcription. KS15 inhibits the interactions between CRYs and BMAL1, resulting in the enhanced transcriptional activity in the core loop of the molecular circadian clock ^[1] .
KS15 directly binds to the C-terminal region of cryptochromes (CRYs: CRY1 and CRY2) and enhances E-box-mediated transcription ^[2] .
KS15 decreases the speed of cell growth and increased the chemosensitivity of MCF-7 cells to Doxorubicin and Tamoxifen. KS15 exerts an anti-proliferative effect and increases sensitivity to anti-tumor drugs in a specific type of breast cancer ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Liquid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL ( 237.93 mM ; Need ultrasonic)

H ₂ O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3793 mL	11.8963 mL	23.7925 mL
5 mM	0.4759 mL	2.3793 mL	4.7585 mL
10 mM	0.2379 mL	1.1896 mL	2.3793 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: 2.5 mg/mL (5.95 mM); Suspended solution; Need ultrasonic
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: 2.5 mg/mL (5.95 mM); Suspended solution; Need ultrasonic
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (5.95 mM); Clear solution

* All of the co-solvents are available by MCE.