[CAS NO. 1233533-04-4] kbNB142-70
PRODUCTS SPECIFICATIONS [1233533-04-4]
DESCRIPTION [1233533-04-4]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 251.32 |
| Molecular Formula | C11H9NO2S2 |
| SMILES | O=C1NCCSC2=C1SC3=CC=C(O)C=C32 |
Summary
IC50 & Target
|
PKD1 28.3 nM (IC 50 ) |
PKD3 53.2 nM (IC 50 ) |
PKD2 58.7 nM (IC 50 ) |
In Vitro
kb NB 142-70 is a potent PKD inhibitor, with IC 50 s of 28.3, 58.7 and 53.2 nM for PKD1, PKD2, and PKD3, respectively. kb NB 142-70 also inhibits Ser 916 phosphorylation of PKD1 (IC 50 , 2.2 ± 0.6 μM) in LNCaP cells. Moreover, kb NB 142-70 is cytotoxic against PC3 cells with an EC 50 of 8.025 μM [1] . kb NB 142-70 (0-5 μM) concentration-dependently prevents ANG II-induced phosphorylation of HDAC4 at Ser 246 and Ser 632 , HDAC5 at Ser 259 and Ser 498 , and HDAC7 at Ser 155 in IEC-18 cells. In addition, kb NB 142-70 (3.5 μM) also suppresses HDAC4, HDAC5, and HDAC7 phosphorylation in IEC-18 cells stimulated with ANG II for 0-240 min or with vasopressin, lysophosphatidic acid (LPA), or phorbol 12,13-dibutyrate (PDBu) [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 46.67 mg/mL ( 185.70 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.9790 mL | 19.8950 mL | 39.7899 mL |
| 5 mM | 0.7958 mL | 3.9790 mL | 7.9580 mL |
| 10 mM | 0.3979 mL | 1.9895 mL | 3.9790 mL |
References
- [1] Lavalle CR, et al. Novel protein kinase D inhibitors cause potent arrest in prostate cancer cell growth and motility. BMC Chem Biol. 2010 May 5;10:5.
- [2] James Sinnett-Smith, et al. Protein kinase D1 mediates class IIa histone deacetylase phosphorylation and nuclear extrusion in intestinal epithelial cells: role in mitogenic signaling. Am J Physiol Cell Physiol. 2014 May 15; 306(10): C961-C971.