[CAS NO. 1247819-59-5] P22077

Ships within

Stock

Price

Qty

Total

$0.00

Please click "REQUEST A QUOTE" button if you need other sizes or custom synthesis

request a quote

If there is no stock, or you need other sizes or custom synthesis, please:

PRODUCTS SPECIFICATIONS [1247819-59-5]

Catalog

HY-13865

Brand

MCE

CAS

1247819-59-5

DESCRIPTION [1247819-59-5]

Overview

MDL	MFCD22580421
Molecular Weight	315.32
Molecular Formula	C12H7F2NO3S2
SMILES	CC(C1=CC([N+]([O-])=O)=C(SC2=CC=C(F)C=C2F)S1)=O

For research use only. We do not sell to patients.

11 Publications Citing Use of MCE

Summary

P 22077 is a cell-permeable ubiquitin-specific protease 7 (USP7) inhibitor with an EC ₅₀ of 8.01 μM. P 22077 also inhibits USP47 with an EC ₅₀ of 8.74 μM.

IC50 & Target

EC50: 8.01 μM (USP7), 8.74 μM (USP47) ^[1]

In Vitro

P 22077 is an inhibitor of USP7 and DUB USP47, with EC ₅₀ s of 8.01 μM and 8.74 μM, respectively. P 22077 (15-45 μM) inhibits a much smaller subset of DUBs. P 22077 (25 μM) causes DUBs inhibition in HEK293T cells ^[1] . P 22077 (0-20 μM) greatly reduces the cell viability of Neuroblastoma (NB) cells including IMR-32, NGP, CHLA-255, and SH-SY5Y cells but without NB-19 and SK-N-AS cells. P 22077 (10 μM) increases p53 activity and induces apoptosis in p53 wild-type and HDM2-expressing NB cells. P 22077 (5 μM) enhances the cytotoxic effect of Dox and VP-16 on NB cells, and enhances Dox- and VP-16-induced p53-mediated apoptosis ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

P 22077 (15 mg/kg, i.p. 21 days) shows potent antitumor activities in an xenograft mouse model bearing IMR-32-derived tumors; P 22077 also exhibits antitumor effects after treatment at 10 mg/kg for 14 days in mice bearing SH-SY5Y-derived tumors, and at 20 mg/kg for 12 days in mice bearing NGP-derived tumors ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL ( 158.57 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1714 mL	15.8569 mL	31.7138 mL
5 mM	0.6343 mL	3.1714 mL	6.3428 mL
10 mM	0.3171 mL	1.5857 mL	3.1714 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (7.93 mM); Clear solution

* All of the co-solvents are available by MCE.