[CAS NO. 1263131-92-5] MRT-83
PRODUCTS SPECIFICATIONS [1263131-92-5]
DESCRIPTION [1263131-92-5]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 538.59 |
| Molecular Formula | C31H30N4O5 |
| SMILES | COC1=C(OC)C(OC)=CC(C(NC(NC2=CC(NC(C3=CC=C(C4=CC=CC=C4)C=C3)=O)=C(C)C=C2)=N)=O)=C1 |
Summary
IC50 & Target
Smo [1] .
In Vitro
MRT-83 displays full antagonist properties with an IC 50 (~3 nM) for inhibiting ShhN (3 nM)-induced proliferation of rat GCPs. MRT-83 also blocks SAG (0.01 μM)-induced proliferation of GCPs (IC 50 ~6 nM). MRT-83 blocks BC binding to HEK-hSmo cells in a dose-dependent manner with an IC 50 of 4.6 nM. MRT-83 abrogates BC binding to cells expressing mouse Smo with an IC 50 of 14 nM, which is in good correlation with its IC 50 in the Shh-light2 and alkaline phosphatase assays [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Animals treated with ShhN in the presence of MRT-83 are as healthy as those of the other groups but up-regulation of Ptc transcription in the SVZ of these animals is no longer observed in agreement with a complete inhibition of ShhN-mediated effects (8.7±2.4 Ptc + cells/section, n=9) and is not different from vehicle-mediated effects. MRT-83 but not MRT-36 antagonizes the up-regulation of Ptc transcription induced by ShhN in vivo in the SVZ of the LV [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 250 mg/mL ( 464.17 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.8567 mL | 9.2835 mL | 18.5670 mL |
| 5 mM | 0.3713 mL | 1.8567 mL | 3.7134 mL |
| 10 mM | 0.1857 mL | 0.9283 mL | 1.8567 mL |
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Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.08 mg/mL (3.86 mM); Clear solution