[CAS NO. 1265917-14-3] S1RAhydrochloride
PRODUCTS SPECIFICATIONS [1265917-14-3]
DESCRIPTION [1265917-14-3]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 373.88 |
| Molecular Formula | C20H24ClN3O2 |
| SMILES | CC1=CC(OCCN2CCOCC2)=NN1C3=CC(C=CC=C4)=C4C=C3.[H]Cl |
1 Publications Citing Use of MCE
Summary
S1RA (E-52862) hydrochloride is an orally active and selective sigma-1 receptor ( σ1R ) antagonist with a K i value of 17 nM. S1RA hydrochloride shows good selectivity against σ2R (K i >1000 nM). S1RA hydrochloride is a human 5-HT2B receptor antagonist with an IC 50 value of 4.7 μM. S1RA hydrochloride inhibits neuropathic pain and activity-induced spinal sensitization [1] [2] .
IC50 & Target
|
Sigma 1 Receptor |
In Vitro
S1RA hydrochloride (0.1-10000 nM) shows high affinity to human and guinea pig σ1Rs with K
i
values of 17 and 23.5 nM, respectively
[1]
.
S1RA hydrochloride (0.1-10000 nM) shows affinity to human σ2R with K
i
values of 9.3 μM
[1]
.
S1RA hydrochloride shows affinity to human 5-HT2B receptor with a K
i
value of 328 nM and an IC
50
value of 4.7 μM
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
S1RA hydrochloride (i.p. 16-80 mg/kg or oral administration 32-128 mg/kg; once) dose-dependently inhibits formalin-induced nociception, capsaicin-induced mechanical hypersensitivity and sciatic nerve injury-induced mechanical and thermal hypersensitivity of mice [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Male CD1 mice [1] |
| Dosage: | 32, 64, 128 and 160 mg/kg |
| Administration: | Intraperitoneal injection; 32, 64, 128 and 160 mg/kg, once |
| Result: | Reduced the permanence time on the rotating rod in rotarod test. |
| Animal Model: | Male CD1 mice with formalin-evoked nociception [1] |
| Dosage: | 20, 40 and 80 mg/kg |
| Administration: | Intraperitoneal injection; 20, 40 and 80 mg/kg, once |
| Result: | Dose-dependently showed analgesic effect on of formalin-evoked nociceptive behaviours of mice. |
| Animal Model: | Male CD1 mice with capsaicin-induced mechanical hypersensitivity [1] |
| Dosage: | 16, 32 and 64 mg/kg (i.p.); 32, 64 and 128 mg/kg (oral administration) |
| Administration: | Intraperitoneal injection or oral administration; 16, 32 and 64 mg/kg (i.p.); 32, 64 and 128 mg/kg (oral administration), once |
| Result: | Dose-dependently reversed capsaicin-induced mechanical hypersensitivity in mice. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL ( 267.47 mM ; Need ultrasonic)
H 2 O : 16.67 mg/mL ( 44.59 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.6747 mL | 13.3733 mL | 26.7465 mL |
| 5 mM | 0.5349 mL | 2.6747 mL | 5.3493 mL |
| 10 mM | 0.2675 mL | 1.3373 mL | 2.6747 mL |
-
1.
Add each solvent one by one: PBS
Solubility: 110 mg/mL (294.21 mM); Clear solution; Need ultrasonic
-
2.
-
3.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (6.69 mM); Clear solution
-
4.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (6.69 mM); Clear solution
References
- [1] Romero L, et al. Pharmacological properties of S1RA, a new sigma-1 receptor antagonist that inhibits neuropathic pain and activity-induced spinal sensitization. Br J Pharmacol. 2012 Aug;166(8):2289-306.
- [2] Vidal-Torres A, et al. Effects of the selective sigma-1 receptor antagonist S1RA on formalin-induced pain behavior and neurotransmitter release in the spinal cord in rats. J Neurochem. 2014 Jan 3.