[CAS NO. 133059-99-1] ZD7288
PRODUCTS SPECIFICATIONS [133059-99-1]
DESCRIPTION [133059-99-1]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 292.81 |
| Molecular Formula | C15H21ClN4 |
| SMILES | [H]Cl.C/N=C1C=C(N(CC)C2=CC=CC=C2)N=C(C)N/1C |
4 Publications Citing Use of MCE
Summary
ZD7288 (ICI D7288) is a selective hyperpolarization-activated cyclic nucleotide-gated ( HCN ) channel blocker [1] .
IC50 & Target
HCN channel [1]
In Vitro
ZD7288 is a selective hyperpolarization-activated cyclic nucleotide-gated (HCN) channel blocker. ZD7288 inhibits glutamate release in a concentration-dependent manner. After incubation with 1, 5 and 50 µM ZD7288 for 24 hours, glutamate content in extracellular fluid is decreased to 69.0±2.8%, 31.4±2.0% and 4.4±0.3%, respectively (P<0.01, vs. DMEM/F12 group [100.2±4.2%]). After incubation with ZD7288 (25, 50, or 100 µM) for 20 minutes, 50 µM glutamate-induced [Ca 2+ ] i rises are attenuated to 59.2±2.7%, 41.4±2.3% and 21.0±1.4%, respectively glutamate (P<0.01, vs. 50 µM glutamate group) [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Application of ZD7288 0.1 µM at 5 minutes before high-frequency stimulation significantly decreases the amplitude of field excitatory postsynaptic potentials (fEPSPs), and this inhibitory effect is maintained throughout the recording period. Application of 0.1 µM ZD7288 30 minutes after high-frequency stimulation almost completely reverses the established long-term potentiation (LTP). Following application of ZD7288 (0.1 µM) 5 minutes before high-frequency stimulation, glutamate content is reduced to 74.9±8.0% (P<0.05, vs. normal saline group). Furthermore, application of 0.1 µM ZD7288 30 minutes after high-frequency stimulation markedly decreases the glutamate content to 77.0%±9.4% (P<0.05, vs. normal saline group) [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 50 mg/mL ( 170.76 mM ; Need ultrasonic)
H 2 O : ≥ 50 mg/mL ( 170.76 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.4152 mL | 17.0759 mL | 34.1518 mL |
| 5 mM | 0.6830 mL | 3.4152 mL | 6.8304 mL |
| 10 mM | 0.3415 mL | 1.7076 mL | 3.4152 mL |
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1.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (341.52 mM); Clear solution; Need ultrasonic
Synonyms