[CAS NO. 136171-87-4] Lobetyol
PRODUCTS SPECIFICATIONS [136171-87-4]
DESCRIPTION [136171-87-4]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 234.29 |
| Molecular Formula | C14H18O3 |
| SMILES | C/C=C/C#CC#CC(O)C(O)/C=C/CCCO |
Summary
IC50 & Target
|
Bax |
Bcl-2 |
In Vitro
Lobetyol (0-100 μg/mL, 0-48 h) leads to a dose-and time-dependent proliferation inhibition in MKN45 cells
[1]
.
Lobetyol (0-100 μg/mL, 48 h) induces
apoptosis
and cell cycle arrest in a time- and dose-dependent manner in MKN45 cells
[1]
.
Lobetyol (0-100 μg/mL, 48 h) increases the expression level of Bax, P53, and decreased the expression level of Bcl-2
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [1]
| Cell Line: | MKN45 cells |
| Concentration: | 0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL |
| Incubation Time: | 12 h, 24 h, 48 h |
| Result: | Showed a dose-and time-dependent proliferation inhibition in MKN45 cells, with an IC 50 of 71.47 ± 4.29 μg/mL at 48 h. Exhibited more safety on L02 and HEK293 human normal cell lines. |
Apoptosis Analysis [1]
| Cell Line: | MKN45 cells |
| Concentration: | 0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL |
| Incubation Time: | 48 h |
| Result: | Increased the apoptotic population in MKN45 cells in a dose-dependent manner. Flow cytometic assay of Annexin-V/PI revealed apoptotic populations under increasing dose of lobetyol ranging from 0 μg/mL, 50 μg/mL, 75 μg/mL, to 100 μg/mL are 5.5%, 13.74%, 27.32% to 31.57%, respectively. |
Western Blot Analysis [1]
| Cell Line: | MKN45 cells |
| Concentration: | 0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL |
| Incubation Time: | 12 h, 24 h, 48 h |
| Result: | Increased the expression level of Bax, P53, and decreased the expression level of Bcl-2. The release of cytochrome c was detected, followed by caspase-9 and -3 activations. |
In Vivo
Lobetyol (0-10 mg/kg, Once per 3 days) suppresses tumor growth in MKN45 nude models [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | BALB/C nude mice (6 weeks-old, injected intraperitoneally with MKN45 cells) [1] |
| Dosage: | 0, 2, 5, 10 mg/kg |
| Administration: | Once per 3 days |
| Result: | Suppressed tumor growth in MKN45 nude models. The protein levels of Ki67 in MKN45 cells were significantly decreased. Meanwhile, cleaved-caspase-3 positive MKN45 cells were found increased with the tendency of lobetyol concentrations in the study in vivo. |
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
References
- [1] Shen J, et al. Lobetyol activate MAPK pathways associated with G1/S cell cycle arrest and apoptosis in MKN45 cells in vitro and in vivo. Biomed Pharmacother. 2016 Jul;81:120-127.
- [2] Xie Q, et al. The in vitro/in vivo metabolic pathways analysis of lobetyol, lobetyolin, and lobetyolinin, three polyacetylenes from Codonopsis Radix, by UHPLC-Q/TOF-MS and UHPLC-MS/MS. J Pharm Biomed Anal. 2023 Jan 20;223:115140.
Synonyms