[CAS NO. 1390654-28-0] KRN2bromide
PRODUCTS SPECIFICATIONS [1390654-28-0]
DESCRIPTION [1390654-28-0]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 524.38 |
| Molecular Formula | C27H23BrFNO4 |
| SMILES | COC1=C(OC)C2=C[N+]3=C(C(C(CC3)=C4)=CC5=C4OCO5)C(CC6=CC=CC=C6F)=C2C=C1.[Br-] |
1 Publications Citing Use of MCE
Summary
KRN2 (bromide) is a selective inhibitor of nuclear factor of activated T cells (NFAT5) , with an IC 50 of 0.1 μM.
IC50 & Target
IC50: 100 nM (NFAT5) [1]
In Vitro
KRN2 selectively suppresses the expression of pro-inflammatory genes, including Nos2 and Il6, without hampering high-salt-induced NFAT5 and its target gene expressions
[1]
.
KRN2 dose-dependently inhibits the NF-κB p65 binding to Nfat5 promoter 1 and directly blocks the interaction between NF-κB p65 and its DNA binding sequence in the upstream site (base pairs -3000 to +1) of
Nfat5
exon 1
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
KRN2 (3 mg/kg, i.p., daily for 2 weeks) effectively suppresses AIA in which innate immune cells play a predominant role
[1]
.
KRN2 (3 mg/kg, i.p., daily) effectively suppresses CIA as well as AIA in mice, decreasing the production of pro-inflammatory cytokines and autoantibodies as well as macrophage infiltration
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | 8-week-old C57BL/6 mice injected intradermally with 2 mg of complete Freund's adjuvant [1] . |
| Dosage: | 3 mg/kg. |
| Administration: | Peritoneally (i.p.) daily for 2 weeks. |
| Result: | Effective in suppressing AIA. |
| Animal Model: | 8-week-old DBA/1Jmice immunized with bovine type II collagen [1] . |
| Dosage: | 3 mg/kg. |
| Administration: | Peritoneally (i.p.) daily. |
| Result: | Effective in suppressing CIA as well as AIA in mice, decreasing the production of pro-inflammatory cytokines and autoantibodies as well as macrophage infiltration. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 18.33 mg/mL ( 34.96 mM ; Need ultrasonic)
H 2 O : 1.1 mg/mL ( 2.10 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.9070 mL | 9.5351 mL | 19.0701 mL |
| 5 mM | 0.3814 mL | 1.9070 mL | 3.8140 mL |
| 10 mM | 0.1907 mL | 0.9535 mL | 1.9070 mL |