[CAS NO. 151-18-8] β-Aminopropionitrile
PRODUCTS SPECIFICATIONS [151-18-8]
DESCRIPTION [151-18-8]
Overview
| MDL | MFCD00014820 |
|---|---|
| Molecular Weight | 70.09 |
| Molecular Formula | C3H6N2 |
| SMILES | N#CCCN |
Summary
IC50 & Target
|
Lysyl Oxidase |
Human Endogenous Metabolite |
In Vitro
β-Aminopropionitrile (BAPN) normalizes the expression of GLUT4 and adiponectin, and improves glucose uptake in an in vitro model of insulin resistance
[1]
.
β-Aminopropionitrile (500 μM; 72 h) blocks the hypoxia-induced EMT morphological and marker protein changes, and inhibits invasion and migration capacities of cervical carcinoma cells in vitro
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | 3T3-L1 adipocytes |
| Concentration: | 200 μM with 1.15 nM and 2.87 nM TNFα |
| Incubation Time: | 72 h |
| Result: | TNFα reduced expression of GLUT4 and adiponectin, and increased SOCS3 protein levels in these cells. And these effects were prevented. |
Cell Invasion Assay [2]
| Cell Line: | HeLa and SiHa cells |
| Concentration: | 500 μM |
| Incubation Time: | 72 h |
| Result: | Significantly reduced hypoxia-elicited cell invasion in both cell models. |
Cell Migration Assay [2]
| Cell Line: | HeLa and SiHa cells |
| Concentration: | 500 μM |
| Incubation Time: | 72 h |
| Result: | Decreased hypoxia-induced migration from 180 and 240% to 60 and 70% in HeLa and SiHa cells, respectively. |
Western Blot Analysis [2]
| Cell Line: | HeLa and SiHa cells |
| Concentration: | 500 μM |
| Incubation Time: | 72 h |
| Result: | Effectively prevented hypoxia-induced downregulation of E-cadherin and strongly inhibited hypoxia-induced upregulation of α-SMA and vimentin. |
In Vivo
β-Aminopropionitrile (BAPN) (100 mg/kg/day; p.o.; 6 weeks) reduces body weight gain and improves the metabolic profile in diet-induced obesity in rats
[1]
.
β-Aminopropionitrile monofumarate (1 g/kg/day; p.o.; 4 weeks) induces thoracic aortic dissection in C57BL/6 mice
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Male Wistar rats of 150 g, high-fat diet (HFD) model [1] |
| Dosage: | 100 mg/kg/day |
| Administration: | In the drinking water, 6 weeks |
| Result: | Significantly prevented the rise in body weight in HFD rats, but not in animals that were fed a standard diet. Reduced the increase in the weight of white adipose tissue (both epididymal and lumbar) in obese animals and attenuated their enhanced adiposity. Improved fasted glucose and insulin levels and consequently reduced HOMA index in the HFD group. Improved insulin signalling in adipose tissue from obese animals. |
| Animal Model: | C57BL/6 mice [3] |
| Dosage: | 1 g/kg/day |
| Administration: | In the drinking water, 4 weeks |
| Result: | Induce thoracic aortic dissection (TAD) in all mice with 24 h of Ang II infusion. Caused 87% of C57BL/6 mice to develop TAD without Ang II. |
Appearance
Liquid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : ≥ 100 mg/mL ( 1426.74 mM )
H 2 O : 50 mg/mL ( 713.37 mM ; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 14.2674 mL | 71.3369 mL | 142.6737 mL |
| 5 mM | 2.8535 mL | 14.2674 mL | 28.5347 mL |
| 10 mM | 1.4267 mL | 7.1337 mL | 14.2674 mL |
-
1.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (1426.74 mM); Clear solution; Need ultrasonic
-
2.
-
3.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 3.25 mg/mL (46.37 mM); Clear solution
-
4.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 3.25 mg/mL (46.37 mM); Clear solution
References
- [1] Miana M, et al. The lysyl oxidase inhibitor β-aminopropionitrile reduces body weight gain and improves the metabolic profile in diet-induced obesity in rats. Dis Model Mech. 2015 Jun;8(6):543-51.
- [2] Yang X, et al. Inactivation of lysyl oxidase by β-aminopropionitrile inhibits hypoxia-induced invasion and migration of cervical cancer cells. Oncol Rep. 2013 Feb;29(2):541-8.
- [3] Ren W, et al. β-Aminopropionitrile monofumarate induces thoracic aortic dissection in C57BL/6 mice. Sci Rep. 2016 Jun 22;6:28149.
Synonyms