[CAS NO. 160369-77-7] Fomivirsen sodium
PRODUCTS SPECIFICATIONS [160369-77-7]
DESCRIPTION [160369-77-7]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 7122.09 |
| Molecular Formula | - |
| SMILES | [Fomivirsen (sodium)] |
Summary
Fomivirsen (ISIS-2922) sodium is an antisense 21 mer phosphorothioate oligonucleotide. Fomivirsen sodium is an antiviral agent that is used cytomegalovirus retinitis (CMV) research, incluiding in AIDs. Fomivirsen sodium binds to and degrades the mRNAs encoding CMV immediate-early 2 protein, thus inhibiting virus proliferation [1] [2] .
In Vitro
Antisense oligonucleotide acts a complementary nucleotide to the messenger RNA of the major immediate-early region proteins of human cytomegalovirus, it disrupts the replication of the virus through an antisense mechanism
[1]
.
Fomivirsen is 21-mer phosphorothioate ASO with CpG motif near its 5′ end, with the sequence 5′-GCG TTT GCT CTT CTT CTT GCG-3′, resulting in mRNA degradation by RNase H-mediated mechanism
[2]
.
Fomivirsen inhibits CMV replication in human retinal pigment epithelial (RPE) cells and in human fibroblast cells (MRC-5 cells) in a dose-dependent manner. The mean IC
50
are 0.03 µM for RPE cells and 0.2 µM for MRC5 cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date | Phase |
|---|---|---|---|---|
| NCT00002187 | Ionis Pharmaceuticals, Inc.|NIH AIDS Clinical Trials Information Service |
Cytomegalovirus Retinitis|HIV Infections
|
Not Applicable | |
| NCT00002356 | Ionis Pharmaceuticals, Inc.|NIH AIDS Clinical Trials Information Service |
Cytomegalovirus Retinitis|HIV Infections
|
Phase 2 | |
| NCT00002355 | Ionis Pharmaceuticals, Inc.|NIH AIDS Clinical Trials Information Service |
Cytomegalovirus Retinitis|HIV Infections
|
Not Applicable | |
| NCT00002156 | Ionis Pharmaceuticals, Inc.|NIH AIDS Clinical Trials Information Service |
Cytomegalovirus Retinitis|HIV Infections
|
Phase 2 |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
H 2 O : 100 mg/mL ( 14.04 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 0.1404 mL | 0.7020 mL | 1.4041 mL |
| 5 mM | 0.0281 mL | 0.1404 mL | 0.2808 mL |
| 10 mM | 0.0140 mL | 0.0702 mL | 0.1404 mL |
-
1.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (14.04 mM); Clear solution; Need ultrasonic
References