[CAS NO. 1681056-61-0] OSMI-1

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PRODUCTS SPECIFICATIONS [1681056-61-0]

Catalog

HY-119738

Brand

MCE

CAS

1681056-61-0

DESCRIPTION [1681056-61-0]

Overview

MDL	MFCD30187585
Molecular Weight	563.64
Molecular Formula	C28H25N3O6S2
SMILES	O=C(N(CC1=CC=CS1)CC2=CC=CO2)[C@H](NS(C3=CC(C=C4)=C(C=C3)NC4=O)(=O)=O)C5=CC=CC=C5OC

For research use only. We do not sell to patients.

3 Publications Citing Use of MCE

Summary

OSMI-1 is a cell-permeable O-GlcNAc transferase (OGT) inhibitor with an IC ₅₀ value of 2.7 μM. OSMI-1 inhibits protein O-linked N-acetylglucosamine (O-GlcNAcylation) in several mammalian cell lines without qualitatively altering cell surface N- or O-linked glycans ^[1] ^[2] .

IC50 & Target

IC50: 2.7 μM (O-GlcNAc transferase) ^[1]

In Vitro

OSMI-1 (50 μM; 24 hours; CHO cells) treatment decreases the viability by about 50% after 24 hours ^[1] .
OSMI-1 (10-100 μM; 24 hours; CHO cells) treatment reduces global O-linked N-acetylglucosamine (O-GlcNAcylation) a dose-dependent manner. OSMI-1 inhibits OGT activity in cells ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay ^[1]

Cell Line:	CHO cells
Concentration:	50 μM
Incubation Time:	24 hours
Result:	Viability decreased by about 50% after 24 hours.

Western Blot Analysis ^[1]

Cell Line:	CHO cells
Concentration:	10 μM, 25 μM, 50 μM, 100 μM
Incubation Time:	24 hours
Result:	Reduced global OGlcNAcylation in a dose-dependent manner.

In Vivo

Mammalian and zebrafish toxicity profiles are strikingly similar, and zebrafish can usually serve as an intermediate step between cell-based evaluation and conventional animal testing. The zebrafish model is used to investigate OSM1-1 acute toxicity in vivo. The LC ₅₀ of OSM1-1 is 0.031 mg/mL (56 μM, 12 h) and 0.025 mg/mL (45 μM, 24 h) in zebrafish model ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL ( 177.42 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7742 mL	8.8709 mL	17.7418 mL
5 mM	0.3548 mL	1.7742 mL	3.5484 mL
10 mM	0.1774 mL	0.8871 mL	1.7742 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: 2.08 mg/mL (3.69 mM); Suspended solution; Need ultrasonic
2.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.08 mg/mL (3.69 mM); Clear solution

* All of the co-solvents are available by MCE.

References

Synonyms

Benzeneacetamide, α-[[(1,2-dihydro-2-oxo-6-quinolinyl)sulfonyl]amino]-N-(2-furanylmethyl)-2-methoxy-N-(2-thienylmethyl)-, (αR)-

(αR)-α-[[(1,2-Dihydro-2-oxo-6-quinolinyl)sulfonyl]amino]-N-(2-furanylmethyl)-2-methoxy-N-(2-thienylmethyl)benzeneacetamide

OSMI 1