[CAS NO. 1808288-51-8] CHMFL-BMX-078
PRODUCTS SPECIFICATIONS [1808288-51-8]
DESCRIPTION [1808288-51-8]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 625.67 |
| Molecular Formula | C33H35N7O6 |
| SMILES | O=C(C1=CN=C(NC2=CC=C(C)C(NC(C=C)=O)=C2)N=C1NC)NC3=CC(NC(C4=CC(OC)=C(OC)C(OC)=C4)=O)=CC=C3C |
1 Publications Citing Use of MCE
Summary
CHMFL-BMX-078 is a highly potent and selective type II irreversible BMX kinase inhibitor with an IC 50 of 11 nM.
IC50 & Target
IC50: 11 nM (BMX) [1]
In Vitro
Bone marrow kinase in the X chromosome (BMX, also called ETK) is a nonreceptor tyrosine kinase involved in tumorigenicity, cell motility, adhesion, angiogenesis, proliferation, and differentiation. CHMFL-BMX-078 exhibits an IC 50 of 11 nM by formation of a covalent bond with cysteine 496 residue in the DFG-out inactive conformation of BMX. It displays a high selectivity profile against the 468 kinases/mutants in the KINOMEscan evaluation and achieves at least 40-fold selectivity over BTK kinase (IC 50 =437 nM). For inactive state of BMX kinase, CHMFL-BMX-078 displays a binding K d of 81 nM, while for the active state of BMX kinase, it exhibits a binding K d of 10200 nM. CHMFL-BMX-078 exhibits antiproliferative effects against BaF3-TEL-BMX cells (GI 50 =0.016 μM) and selectivity over parental BaF3 cells. CHMFL-BMX-078 is about 80-fold more potent against BMX wt (EC 50 =5.8 nM) than C496S mutant (EC 50 =459 nM) for the inhibition of BMX total tyrosine phosphorylation. CHMFL-BMX-078 would serve as a useful pharmacological tool to elucidate the detailed mechanism of BMX mediated signaling pathways [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
CHMFL-BMX-078 exhibits a short half-life (T 1/2 =0.80 h) in iv injection. CHMFL-BMX-078 also displays an acceptable C max (13565.23 ng/mL) and AUC 0-t (1386.41 ng/mL h) in iv injection. However, it is not absorbed by oral administration, indicating that this compound could be administrated through iv or ip injection when used as a research tool [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 30 mg/mL ( 47.95 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.5983 mL | 7.9914 mL | 15.9829 mL |
| 5 mM | 0.3197 mL | 1.5983 mL | 3.1966 mL |
| 10 mM | 0.1598 mL | 0.7991 mL | 1.5983 mL |
-
1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (4.00 mM); Clear solution