[CAS NO. 1883545-60-5] CRT0066101dihydrochloride
PRODUCTS SPECIFICATIONS [1883545-60-5]
DESCRIPTION [1883545-60-5]
Overview
| MDL | MFCD30182249 |
|---|---|
| Molecular Weight | 411.33 |
| Molecular Formula | C18H24Cl2N6O |
| SMILES | OC1=CC=C(C2=CN(C)N=C2)C=C1C3=NC=CC(NC[C@H](N)CC)=N3.[H]Cl.[H]Cl |
1 Publications Citing Use of MCE
Summary
IC50 & Target
|
PKD1 1 nM (IC 50 ) |
PKD3 2 nM (IC 50 ) |
PKD2 2.5 nM (IC 50 ) |
In Vitro
CRT0066101 specifically blocks PKD1/2 activity and does not suppress PKCα/PKCβ/PKCε activity in multiple cancer cell types including A549 (lung) and MiaPaCa-2 (pancreas). CRT0066101 significantly inhibits Panc-1 cell proliferation with an IC 50 value of 1 µM. Treatment with CRT0066101 results in a 6-10 fold induction of apoptosis in Panc-1 cells. CRT0066101 significantly reduces cell proliferation of Colo357, Panc-1, MiaPaCa-2, and AsPC-1 cells but has a modest effect in Capan-2 cells. CRT0066101 (5 µM) blocks both the basal and NT-induced pS916-PKD1/2 (activated PKD1/2) in Panc-1 and Panc-28 cells. CRT0066101 reduces PKD-dependent NF-κB activation and NF-κB-dependent gene expressions in Panc-1 [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Optimal therapeutic concentrations (8 µM) of CRT0066101 are detectable 6 h after oral administration of this drug. CRT0066101 given orally (80 mg/kg/day) for 28 days significantly abrogates PaCa growth in Panc-1 subcutaneous xenograft model. Activated PKD1/2 expression in the treated tumor-explants is significantly inhibited with peak tumor concentration (12 µM) of CRT0066101 achieved within 2 h after oral administration. Further, CRT0066101 given orally (80 mg/kg/day) for 21 days in Panc-1 orthotopic model potently blocks tumor growth in vivo . CRT0066101 significantly reduces Ki-67 + proliferation index, increases TUNEL+ apoptotic cells (p<0.05), and abrogates expression of NF-κB-dependent proteins including cyclin D1, survivin, and cIAP-1 [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H 2 O : 50 mg/mL ( 121.56 mM ; Need ultrasonic)
DMSO : 11.36 mg/mL ( 27.62 mM ; ultrasonic and warming and heat to 60°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.4311 mL | 12.1557 mL | 24.3114 mL |
| 5 mM | 0.4862 mL | 2.4311 mL | 4.8623 mL |
| 10 mM | 0.2431 mL | 1.2156 mL | 2.4311 mL |
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1.
Add each solvent one by one: PBS
Solubility: 25 mg/mL (60.78 mM); Clear solution; Need ultrasonic
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2.
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3.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 1.14 mg/mL (2.77 mM); Clear solution
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4.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 1.14 mg/mL (2.77 mM); Clear solution