[CAS NO. 19103-54-9] Salvigenin
PRODUCTS SPECIFICATIONS [19103-54-9]
DESCRIPTION [19103-54-9]
Overview
| MDL | MFCD00238678 |
|---|---|
| Molecular Weight | 328.32 |
| Molecular Formula | C18H16O6 |
| SMILES | O=C1C=C(C2=CC=C(OC)C=C2)OC3=CC(OC)=C(OC)C(O)=C13 |
Summary
In Vitro
Salvigenin (0-100 μM, 3 h) protects SH-SY5Y cells against H
2
O
2
-induced cell death
[1]
.
Salvigenin (0-100 μM, 2 h) significantly reduces ROS generation, increases the glutathione level, declines the extent of cell apoptosis and increases autophagy in H
2
O
2
-exposed SH-SY5Y cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | SY5Y cells exposed to H 2 O 2 (300 μM) |
| Concentration: | 10, 25, 50, and 100 μM |
| Incubation Time: | 3 h |
| Result: | Protected SH-SY5Y cells (best in 25 and 50μM) against H 2 O 2 -induced cell death. |
Apoptosis Analysis [1]
| Cell Line: | SY5Y cells exposed to H 2 O 2 (300 μM) |
| Concentration: | 10, 25, 50, and 100 μM |
| Incubation Time: | 2 h |
| Result: | Significantly declined the extent of cell apoptosis induced by H 2 O 2 . |
Western Blot Analysis [1]
| Cell Line: | SY5Y cells exposed to H 2 O 2 (300 μM) |
| Concentration: | 10, 25, 50, and 100 μM |
| Incubation Time: | 2 h |
| Result: | Reduced cleaved caspase-3 and Bax/Bcl-2 ratio. Reduced caspase-12 and calpain levels. Reduced the level of Atg7, Atg12, and LC3-II/ LC3-I. |
In Vivo
Salvigenin (0-9.68 μg/mouse/day; i.p.; 4 or 12 days) shows antitumor and immunomodulatory effects on tumor bearing mice [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | MCF-7 cell bearing inbred female Balb/c mice, aged from six to eight weeks [2] |
| Dosage: | 3.65, 5.85 and 9.68 μg/mouse/day for four days or 12 days |
| Administration: | Intraperitoneal injection |
| Result: | Showed a significant increase in DTH response in a dose-dependent manner. Significantly decreased the rate of tumor growth, increased lymphocyte proliferation index, increased the level of IFN-γ and decreased IL-4 production. Exhibited a significant decrease of the splenic CD4 + CD25 + Foxp 3+ T lymphocytes. |
Appearance
Solid
Source
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 20 mg/mL ( 60.92 mM ; ultrasonic and warming and heat to 60°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.0458 mL | 15.2290 mL | 30.4581 mL |
| 5 mM | 0.6092 mL | 3.0458 mL | 6.0916 mL |
| 10 mM | 0.3046 mL | 1.5229 mL | 3.0458 mL |
-
1.
References
- [1] Rafatian G, et al. Increase of autophagy and attenuation of apoptosis by Salvigenin promote survival of SH-SY5Y cells following treatment with H₂O₂. Mol Cell Biochem. 2012 Dec;371(1-2):9-22.
- [2] Noori S, et al. Antitumor and immunomodulatory effects of salvigenin on tumor bearing mice. Cell Immunol. 2013 Nov-Dec;286(1-2):16-21.
Synonyms