[CAS NO. 1941174-13-5] KMH-233
PRODUCTS SPECIFICATIONS [1941174-13-5]
DESCRIPTION [1941174-13-5]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 587.58 |
| Molecular Formula | C32H25N7O5 |
| SMILES | O=C([C@H](CC1=CC=CC(C(NC2=C(C(C3=CC=C(C=C3)OCC(NC)=O)=C(C4=NC5=CC=CC=C5N24)C#N)C#N)=O)=C1)N)O |
Summary
KMH-233, a potent, reversible and selective l-type amino acid transporter 1 (LAT1) inhibitor, inhibits the uptake of LAT1 substrate, l-leucin (IC 50 =18 μM) as well as cell growth. KMH-233 significantly potentiates the efficacy of Bestatin and Cisplatin even at low concentrations (25 μM) [1] .
In Vitro
KMH-233 is able to inhibit binding and transport of essential neutral amino acids and thus, inhibit the cell growth of cancer cells
[1]
.
KMH-233 shows a significant reduction of cell growth with an IC
50
of 124 µM
[1]
.
KMH-233 is effective and able to potentiate the anti-proliferative efficacy of Bestatin (100 µM) and Cisplatin (100 µM) at a lower concentration of 25 µM, inhibiting cell growth 53% and 50%, respectively
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [1]
| Cell Line: | MCF-7 cells |
| Concentration: | 0.5-1000 μM |
| Incubation Time: | 72 hours |
| Result: | Showed a significant reduction of cell growth with an IC 50 of 124 µM. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 100 mg/mL ( 170.19 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.7019 mL | 8.5095 mL | 17.0190 mL |
| 5 mM | 0.3404 mL | 1.7019 mL | 3.4038 mL |
| 10 mM | 0.1702 mL | 0.8509 mL | 1.7019 mL |
-
1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: 2.5 mg/mL (4.25 mM); Suspended solution; Need ultrasonic