[CAS NO. 1991986-30-1] ML364
PRODUCTS SPECIFICATIONS [1991986-30-1]
DESCRIPTION [1991986-30-1]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 517.54 |
| Molecular Formula | C24H18F3N3O3S2 |
| SMILES | O=C(NC1=NC(C2=CC=CC=C2)=CS1)C3=CC=C(C(F)(F)F)C=C3NS(=O)(C4=CC=C(C)C=C4)=O |
8 Publications Citing Use of MCE
Summary
ML364 is a selective ubiquitin specific peptidase 2 (USP2) inhibitor ( IC 50 =1.1 μM) with anti-proliferative activity, which direct binds to USP2 ( K d =5.2 μM), induces an increase in cellular cyclin D1 degradation and causes cell cycle arrest. ML364 increases the levels of mitochondrial ROS and decreases in the intracellular content of ATP [1] [2] .
In Vitro
ML364 (5-20 μM; 24-48 hours) inhibits LnCAP and MCF7 cells viability in a dose-dependent manner
[1]
.
ML364 (10 μM; 2-24 hours) reduces cyclin D1 protein levels in a time-, dose-, and proteasome-dependent manner in HCT116 cells and Mino cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | LnCAP, MCF7 cells |
| Concentration: | 5, 10, 15, 20 μM |
| Incubation Time: | 24, 48 hours |
| Result: | LnCAP and MCF7 cells showed a decrease in cell viability in a dose-dependent manner. |
Western Blot Analysis [1]
| Cell Line: | HCT116, Mino cells |
| Concentration: | 2, 4, 8, 16, 24 hours |
| Incubation Time: | 10 μM |
| Result: | Reduced cyclin D1 protein levels in a time-, dose-, and proteasome-dependent manner in HCT116 cells and Mino cells. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 33 mg/mL ( 63.76 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.9322 mL | 9.6611 mL | 19.3222 mL |
| 5 mM | 0.3864 mL | 1.9322 mL | 3.8644 mL |
| 10 mM | 0.1932 mL | 0.9661 mL | 1.9322 mL |
References
- [1] Davis MI, et al. Small Molecule Inhibition of the Ubiquitin-specific Protease USP2 Accelerates cyclin D1 Degradation and Leads to Cell Cycle Arrest in Colorectal Cancer and Mantle Cell Lymphoma Models. J Biol Chem. 2016 Nov 18;291(47):24628-24640.
- [2] Hashimoto M, et al. Inhibition of ubiquitin-specific protease 2 causes accumulation of reactive oxygen species, mitochondria dysfunction, and intracellular ATP decrement in C2C12 myoblasts. Physiol Rep. 2019 Jul;7(14):e14193.