[CAS NO. 2093393-05-4] TC13172
PRODUCTS SPECIFICATIONS [2093393-05-4]
DESCRIPTION [2093393-05-4]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 388.40 |
| Molecular Formula | C17H16N4O5S |
| SMILES | O=C1C2=C(N=C(S(C)(=O)=O)N2C)N(CC#CC3=CC(O)=CC=C3)C(N1C)=O |
Summary
TC13172 is a mixed lineage kinase domain-like protein ( MLKL ) inhibitor with an EC 50 value of 2 nM for HT-29 cells [1] .
IC50 & Target
EC50: 2±0.6 nM (MLKL, in HT-29 cells) [1] .
In Vitro
The anti-necroptosis potency of TC13172 is evaluated in the HT-29 cell line, the EC 50 value is 2±0.6 nM. TC13172 has an inhibition potency of 2 nM against cell necroptosis. TC13172 inhibits MLKL by directly binding to Cys-86. TC13172 does not disrupt the phosphorylation of MLKL, but do decrease the level of MLKL in the membrane phase, demonstrating that these MLKL inhibitors block the translocation of MLKL to the cell membrane, thereby protecting cells from necroptosis [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 25 mg/mL ( 64.37 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.5747 mL | 12.8733 mL | 25.7467 mL |
| 5 mM | 0.5149 mL | 2.5747 mL | 5.1493 mL |
| 10 mM | 0.2575 mL | 1.2873 mL | 2.5747 mL |
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Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.25 mg/mL (5.79 mM); Clear solution