[CAS NO. 2095677-20-4] eIF4A3-IN-2
PRODUCTS SPECIFICATIONS [2095677-20-4]
DESCRIPTION [2095677-20-4]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 602.70 |
| Molecular Formula | C25H19Br2ClN4O2 |
| SMILES | O=C(C1=CC=C(Br)C=C1)N2CCN(C(C3=C4N(C=C(Br)C=C4)N=C3)=O)C[C@@H]2C5=CC=C(Cl)C=C5 |
1 Publications Citing Use of MCE
Summary
eIF4A3-IN-2 is a highly selective and noncompetitive eukaryotic initiation factor 4A-3 (eIF4A3) inhibitor with an IC 50 of 110 nM [1] .
IC50 & Target
IC50: 110 nM (eIF4A3) [1]
In Vitro
eIF4A3-IN-2 (compound 2) binds to the allosteric region in eIF4A3 and inhibits in vitro ATPase, helicase, and cellular nonsense-mediated RNA decay (NMD) activities [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Apoptosis Analysis [1]
| Cell Line: | HEK293T cells (transfected with the NMD reporter) luciferase assay |
| Concentration: | 0.3, 1, 3, or 10 μM |
| Incubation Time: | 3 or 6 h |
| Result: | eIF4A3-IN-2 induces an approximately 3.2-fold increase in luciferase activity, indicating that NMD is inhibited by compound 2 [1] . |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 100 mg/mL ( 165.92 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.6592 mL | 8.2960 mL | 16.5920 mL |
| 5 mM | 0.3318 mL | 1.6592 mL | 3.3184 mL |
| 10 mM | 0.1659 mL | 0.8296 mL | 1.6592 mL |
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Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 1.67 mg/mL (2.77 mM); Clear solution