[CAS NO. 2310262-11-2] PDK4-IN-1hydrochloride
PRODUCTS SPECIFICATIONS [2310262-11-2]
DESCRIPTION [2310262-11-2]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 393.87 |
| Molecular Formula | C22H20ClN3O2 |
| SMILES | [H]Cl.O=C1C2=C(C=CC=C2)C(C3=CC=CC(C4=CN(C5CCNCC5)N=C4)=C13)=O |
Summary
PDK4-IN-1 hydrochloride is an anthraquinone derivative and a potent and orally active pyruvate dehydrogenase kinase 4 (PDK4) inhibitor with an IC 50 value of 84 nM. PDK4-IN-1 hydrochloride potently represses cellular transformation and cellular proliferation and induces apoptosis . PDK4-IN-1 hydrochloride has antidiabetic, anticancer and anti-allergic activity [1] .
IC50 & Target
IC50: 84 nM (Pyruvate dehydrogenase kinase 4 (PDK4)) [1]
In Vitro
PDK4-IN-1 (Compound 8c; 50 μM; 0-72 hours; HCT116 and RKO cells) treatment significantly impedes the proliferation of human colon cancer cell lines, HCT116 and RKO. The colony formation efficiency in HCT116 and RKO cells Is significantly reduced after treatment of PDK4-IN-1
[1]
.
PDK4-IN-1 (Compound 8c; 10-50 μM; 24 hours; HCT116 and RKO cells) treatment dose-dependently increased apoptosis
[1]
.
PDK4-IN-1 (Compound 8c; 10 μM; 24 hours; HEK293T cells) treatment inhibits phosphorylation of Ser
232
, Ser
293
, and Ser
300
of PDHE1α
[1]
.
10 μM of PDK4-IN-1 (Compound 8c) significantly increases p-Akt in AML12 cells
[1]
.
PDK4-IN-1 (compound 8c)-induced phosphorylation of p53 on serine 15 is a dose-dependent response in both HCT116 and RKO cells. PDK4-IN-1 decreases the expression of BCL-xL and increases the expression of BAX. Cleavage of PARP1 and caspase 3 are increased by PDK4-IN-1
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | HCT116 and RKO cells |
| Concentration: | 50 μM |
| Incubation Time: | 0 hour, 24 hours, 48 hours, 72hours |
| Result: | Significantly impeded the proliferation of human colon cancer cell lines, HCT116 and RKO. |
Apoptosis Analysis [1]
| Cell Line: | HCT116 and RKO cells |
| Concentration: | 10 μM, 25 μM, 50 μM |
| Incubation Time: | 24 hours |
| Result: | Dose-dependently increased apoptosis. |
Western Blot Analysis [1]
| Cell Line: | HEK293T human embryonic kidney cells |
| Concentration: | 10 μM |
| Incubation Time: | 24 hours |
| Result: | Inhibited phosphorylation of Ser 232 , Ser 293 , and Ser 300 of PDHE1α. |
In Vivo
PDK4-IN-1 (Compound 8c; 100 mg/kg; oral administration; daily; for 1 week; C57BL/6J mice) treatment significantly improves glucose tolerance
[1]
.
Pre-incubation with PDK4-IN-1 (compound 8c) dose-dependently inhibits the release of β-hexosaminidase from IgE/antigen-activated BMMCs, showing that the absorbance values are 0.26, 0.20, and 0.126 in IgE/Ag, 10 μM, and 20 μM PDK4-IN-1-treated BMMCs
[1]
.
The pharmacokinetic (PK) profiles of PDK4-IN-1 (compound 8c) are evaluated in rat. PDK4-IN-1 shows good bioavailability (64%), long half-life (>7 h), and moderate clearance (CL of 0.69) in rats
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | C57BL/6J mice (8-week old) fed with high-fat diet [1] |
| Dosage: | 100 mg/kg |
| Administration: | Oral administration; daily; for 1 week |
| Result: | Significantly improved glucose tolerance. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 125 mg/mL ( 317.36 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.5389 mL | 12.6945 mL | 25.3891 mL |
| 5 mM | 0.5078 mL | 2.5389 mL | 5.0778 mL |
| 10 mM | 0.2539 mL | 1.2695 mL | 2.5389 mL |