[CAS NO. 2376255-48-8] Ac-FLTD-CMK
PRODUCTS SPECIFICATIONS [2376255-48-8]
DESCRIPTION [2376255-48-8]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 569.05 |
| Molecular Formula | C26H37ClN4O8 |
| SMILES | ClCC([C@H](CC(O)=O)NC([C@H]([C@H](O)C)NC([C@H](CC(C)C)NC([C@@H](NC(C)=O)CC1=CC=CC=C1)=O)=O)=O)=O |
2 Publications Citing Use of MCE
Summary
Ac-FLTD-CMK, a gasdermin D (GSDMD)-derived inhibitor, is a specific inflammatory caspases inhibitor. Ac-FLTD-CMK is effective against caspases-1 ( IC 50 of 46.7 nM), caspases-4 ( IC 50 of 1.49 μM), caspases-5 ( IC 50 of 329 nM), and caspases-11 , but not the apoptotic caspases such as caspase-3 [1] .
IC50 & Target
|
Caspase-1 46.7 nM (IC 50 ) |
Caspase-4 1.49 μM (IC 50 ) |
Caspase-5 329 nM (IC 50 ) |
Caspase-11
|
In Vitro
Ac-FLTD-CMK, inhibits GSDMD cleavage by caspases-1, -4, -5, and -11 in vitro, suppresses pyroptosis downstream of both canonical and noncanonical inflammasomes, as well as reduces IL-1β release following activation of the NLRP3 inflammasome in macrophages. By contrast, Ac-FLTD-CMK does not target caspase-3 or apoptotic cell death. Crystal structure of caspase-1 in complex with Ac-FLTD-CMK reveals extensive enzyme-inhibitor interactions involving both hydrogen bonds and hydrophobic contacts [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL ( 175.73 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.7573 mL | 8.7866 mL | 17.5731 mL |
| 5 mM | 0.3515 mL | 1.7573 mL | 3.5146 mL |
| 10 mM | 0.1757 mL | 0.8787 mL | 1.7573 mL |