[CAS NO. 2541792-70-3] ASK1-IN-2
PRODUCTS SPECIFICATIONS [2541792-70-3]
DESCRIPTION [2541792-70-3]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 364.38 |
| Molecular Formula | C19H17FN6O |
| SMILES | O=C(C(N1)=CC2=C1C=C(F)C=C2)NC3=NC(C4=NN=CN4C(C)C)=CC=C3 |
Summary
IC50 & Target
|
ASK1 32.8 nM (IC 50 ) |
In Vitro
ASK1-IN-2 (compound 19) (10 mM; 1 h) inhibits the luciferase reporter activity in AP1-HEK293 cells, with inhibition rate of 95.59% [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
ASK1-IN-2 (25 mg/kg; p.o. daily for 7 d) improves dextran sulphate sodium (DSS)-induced ulcerative colitis (UC) in mice
[1]
.
ASK1-IN-2 (25 mg/kg; p.o. daily for 7 d) blocks ASK1-p38/JNK signaling pathways and reduces inflammatory cytokine levels in DSS-induced mouse colon tissues
[1]
.
ASK1-IN-2 (1 mg/kg; i.v.) shows low clearance (CL=1.38 L/h/kg) and moderate half-life (T
1/2
=1.45 h) in rats
[1]
.
ASK1-IN-2 (10 mg/kg; p.o.) shows high oral exposure (AUC
last
=4517 h•ng/mL), 62.2% oral bioavailability and acceptable terminal half-life (T
1/2
=2.31 h) in rats
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Male ICR mice (18-22 g, 6-8 weeks) were given 3% DSS (w/v) orally in drinking water [1] |
| Dosage: | 25 mg/kg |
| Administration: | P.o. daily for 7 days |
| Result: |
Induced a significant recovery of body weight loss, with an increase of +11.2%.
Decreased the disease activity index (DAI) score about a 2 unit. Significantly prevented colon shortening. Attenuated a severe colonic tissue damage and infiltration of inflammatory cells. |
| Animal Model: | Male SD rats [1] |
| Dosage: | 1 mg/kg for i.v.; 10 mg/kg for p.o. (Pharmacokinetic Analysis) |
| Administration: | I.v. and p.o. administration |
| Result: |
I.v.: CL=1.38 L/h/kg; T
1/2
=1.45 h.
P.o.: AUC last =4517 h•ng/mL; F=62.2%; T 1/2 =2.31 h. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 250 mg/mL ( 686.10 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.7444 mL | 13.7219 mL | 27.4439 mL |
| 5 mM | 0.5489 mL | 2.7444 mL | 5.4888 mL |
| 10 mM | 0.2744 mL | 1.3722 mL | 2.7444 mL |
-
1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.08 mg/mL (5.71 mM); Clear solution