[CAS NO. 258843-62-8] Hoechst33258analog
PRODUCTS SPECIFICATIONS [258843-62-8]
DESCRIPTION [258843-62-8]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 510.59 |
| Molecular Formula | C29H30N6O3 |
| SMILES | O=C(O)CCCOC1=CC=CC(C2=NC3=CC=C(C4=NC5=CC=C(N6CCN(C)CC6)C=C5N4)C=C3N2)=C1 |
1 Publications Citing Use of MCE
Summary
Hoechst 33258 analog is an analogue of the Hoechst dye. Hoechst 33258 analog is a cellular dye that can be used to quantify DNA. Hoechst 33258 analog emits blue fluorescence when bound to dsDNA [1] .
In Vitro
Hoechst 33258 analog is used to detect the presence of nucleic acids in cells. Hoechst 33258 analog specifically to the target DNA sequence and emits blue fluorescence when bound to dsDNA. Hoechst 33258 analog is conjugated to a reporter probe and a signal generator [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : ≥ 51 mg/mL ( 99.88 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.9585 mL | 9.7926 mL | 19.5852 mL |
| 5 mM | 0.3917 mL | 1.9585 mL | 3.9170 mL |
| 10 mM | 0.1959 mL | 0.9793 mL | 1.9585 mL |
References
- [1] a b c "Hoechst Stains". Invitrogren (Molecular Probes).
- [2] Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505.
- [3] Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68.