[CAS NO. 265646-85-3] Emidurdar

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PRODUCTS SPECIFICATIONS [265646-85-3]

Catalog

HY-105917

Brand

MCE

CAS

265646-85-3

DESCRIPTION [265646-85-3]

Overview

MDL	-
Molecular Weight	495.18
Molecular Formula	C16H9BrF6N6O
SMILES	FC(F)(F)C1=CC(C(F)(F)F)=CC(NC(NC2=C(C3=NN=NN3)C=C(Br)C=C2)=O)=C1

For research use only. We do not sell to patients.

1 Publications Citing Use of MCE

[1]Nat Commun. 2021 Jul 22;12(1):4457.

Summary

Endovion (NS3728) is a pharmacological anion channel inhibitor (like chloride channel) and the specific VRAC/VSOAC blocker. Endovion (NS3728) is also an Anoctamin-1 (ANO 1) channel inhibitor ^[1] ^[2] .

IC50 & Target

Chloride channel, VRAC/VSOAC ^[1] .
ANO1 ^[2] .

In Vitro

Endovion (NS3728, 10-100 μM) reduces TNFα-induced apoptosis and increases p53-protein level as well as downstream signaling, e.g., expression of p21 ^Waf1/Cip1 , Bax, Noxa, MDM2, and activation of Caspase-9/-3 in Cisplatin-sensitive cells ^[1] .
Endovion (NS3728, 10 μM) inhibits cell proliferation in Capan-1, AsPC-1 and BxPC-3 cell lines ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis ^[1]

Cell Line:	Wild-type, resistant, and transiently transfected A2780 cells.
Concentration:	10-100 μM.
Incubation Time:	18 or 4.5 h.
Result:	Reduced the maximal taurine rate constant more than 90% compared with the untreated control cells. Resulted in an increased LRRC8A protein expression. Significantly reduceed p53 and ^p21Waf1/Cip1 protein level in A2780WT cells.

Cell Proliferation Assay ^[2]

Cell Line:	Capan-1, AsPC-1, BxPC-3 and H6c7 cell lines.
Concentration:	10 μM.
Incubation Time:	24 h.
Result:	Resulted in the most pronounced inhibition in all cell lines with 77 ± 26 % in Capan-1, 67 ± 9 % in AsPC-1, and 54 ± 8 % in BxPC-3 cells at +67 mV.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL ( 252.43 mM ; Need ultrasonic)

H ₂ O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0195 mL	10.0973 mL	20.1947 mL
5 mM	0.4039 mL	2.0195 mL	4.0389 mL
10 mM	0.2019 mL	1.0097 mL	2.0195 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (4.20 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.08 mg/mL (4.20 mM); Clear solution

* All of the co-solvents are available by MCE.