[CAS NO. 28718-90-3] DAPI dihydrochloride

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PRODUCTS SPECIFICATIONS [28718-90-3]

Catalog

HY-D0814

Brand

MCE

CAS

28718-90-3

DESCRIPTION [28718-90-3]

Overview

MDL	MFCD00012681
Molecular Weight	350.25
Molecular Formula	C16H17Cl2N5
SMILES	N=C(C1=CC2=C(C=C1)C=C(C3=CC=C(C(N)=N)C=C3)N2)N.[H]Cl.[H]Cl

For research use only. We do not sell to patients.

21 Publications Citing Use of MCE

Summary

DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.

In Vitro

DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. DAPI can be used as a DNA-specific probe for flow cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 75 mg/mL ( 214.13 mM ; Need ultrasonic)

H ₂ O : 5.56 mg/mL ( 15.87 mM ; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8551 mL	14.2755 mL	28.5510 mL
5 mM	0.5710 mL	2.8551 mL	5.7102 mL
10 mM	0.2855 mL	1.4276 mL	2.8551 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

* All of the co-solvents are available by MCE.

References

[1] Kapuscinski J, et al. DAPI: a DNA-specific fluorescent probe. Biotech Histochem. 1995 Sep;70(5):220-33.

Synonyms

1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, hydrochloride (1:2)

Indole-6-carboxamidine, 2-(p-amidinophenyl)-, dihydrochloride

1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, dihydrochloride

6-Amidino-2-(4-amidinophenyl)indole dihydrochloride

4′,6-Diamidino-2-phenylindole dihydrochloride

FxCycle Violet

DAPI