[CAS NO. 2975-36-2] Mepazinehydrochloride
PRODUCTS SPECIFICATIONS [2975-36-2]
DESCRIPTION [2975-36-2]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 346.92 |
| Molecular Formula | C19H23ClN2S |
| SMILES | CN1CC(CN2C3=C(C=CC=C3)SC4=CC=CC=C24)CCC1.[H]Cl |
1 Publications Citing Use of MCE
Summary
IC50 & Target
MALT1 [1]
In Vitro
Mepazine (5-20 μM; 4 days) causes a decrease of cell viability in the activated B cell subtype of diffuse large B cell lymphoma (ABCDLBCL) cells, without significantly affecting GCB-DLBCL cells [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | ABC-DLBCL cell lines (HBL1, OCI-Ly3, U2932, TMD8, OCI-Ly10) and GCB-DLBCL cell lines (BJAB, Su-DHL-6, Su-DHL-4) |
| Concentration: | 5, 10, and 20 μM |
| Incubation Time: | 4 days |
| Result: | Caused a decrease of cell viability in the ABC-DLBCL cells HBL1, OCI-Ly3, U2932, and TMD8, without significantly affecting GCB-DLBCL cells. |
In Vivo
Mepazine (16 mg/kg; intraperitoneal administration) interferes with growth and induces apoptosis of ABC-DLBCL cell line OCI-Ly10 in NOD/scid IL-2Rg null (NSG) mice with a murine DLBCL xenogeneic tumor model. Daily administration of Mepazine strongly impairs the expansion of the ABC-DLBCL cell line OCI-Ly10 [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | 6- to 8-week-old female NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice with a murine DLBCL xenogeneic tumor model [1] |
| Dosage: | 400 μg per animal (25 g), corresponding to approximately 16 mg/kg |
| Administration: | Intraperitoneal administration; started 1 or 12 days after transplantation and given continuously every 24 hr; daily application |
| Result: | Daily administration strongly impaired the expansion of the ABC-DLBCL cell line OCI-Ly10. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 50 mg/mL ( 144.13 mM ; Need ultrasonic)
H 2 O : 2.22 mg/mL ( 6.40 mM ; ultrasonic and warming and heat to 60°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.8825 mL | 14.4125 mL | 28.8251 mL |
| 5 mM | 0.5765 mL | 2.8825 mL | 5.7650 mL |
| 10 mM | 0.2883 mL | 1.4413 mL | 2.8825 mL |
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1.
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2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (7.21 mM); Clear solution
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3.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.21 mM); Clear solution
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4.
Add each solvent one by one: PBS
Solubility: 1.25 mg/mL (3.60 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Synonyms