[CAS NO. 312631-87-1] 10074-A4
PRODUCTS SPECIFICATIONS [312631-87-1]
DESCRIPTION [312631-87-1]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 409.29 |
| Molecular Formula | C18H14Cl2N2O3S |
| SMILES | O=C(SCC1=O)N1CC(CN2C3=C(C=C(C=C3)Cl)C4=C2C=CC(Cl)=C4)O |
Summary
10074-A4 is a c-Myc inhibitor . 10074-A4 could bind to c-Myc 370-409 at different sites along the peptide chain. 10074-A4 has anticancer effects [1] [2] .
In Vitro
10074-A4 shows inhibitory activity of HL-60 cells with an IC
50
of 15.1 μM
[1]
.
10074-A4 (25-50 μM; 24 hours) arrests the cell cycle at the S-phase in a dose-dependent manner in HL-60 cells. 10074-A4 inhibits the mRNA level of the c-Myc target genes, CCND2 and CDK4
[1]
.
10074-A4 could bind to c-Myc
370-409
at different sites along the peptide chain and its binding behavior could be described as a ‘ligand cloud’. Even in the bound state, the structure of the c-Myc
370-409
peptide remained a dynamic ensemble. The 10074-A4 ligand bound at different sites throughout the c-Myc
370-409
chain with different strength
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | HL-60 cells |
| Concentration: | 25 μM, 50 μM |
| Incubation Time: | 24 hours |
| Result: | Arrested the cell cycle at the S-phase. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 125 mg/mL ( 305.41 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.4433 mL | 12.2163 mL | 24.4326 mL |
| 5 mM | 0.4887 mL | 2.4433 mL | 4.8865 mL |
| 10 mM | 0.2443 mL | 1.2216 mL | 2.4433 mL |
-
1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.08 mg/mL (5.08 mM); Clear solution
References