[CAS NO. 347323-96-0] ITX3
PRODUCTS SPECIFICATIONS [347323-96-0]
DESCRIPTION [347323-96-0]
Overview
| MDL | MFCD02629172 |
|---|---|
| Molecular Weight | 371.45 |
| Molecular Formula | C22H17N3OS |
| SMILES | O=C(/C1=C\C2=C(C)N(C(C)=C2)C3=CC=CC=C3)N4C(S1)=NC5=CC=CC=C45 |
2 Publications Citing Use of MCE
Summary
IC50 & Target
IC50: 76 μM (TrioN) [1]
In Vitro
ITX3 (5, 10, 25, 50 and 100 μM; 24 h) inhibits TrioN signaling
[1]
.
ITX3 (50 μM; 1 h) specifically inhibits TrioN
[1]
.
ITX3 (100 μM; 36 h) inhibits nerve growth factor-induced neurite outgrowth in PC12 cells
[1]
.
ITX3 (1, 10 and 100 μM) represses Rac1 activity and dose-dependently up-regulates the E-cadherin protein level and phospho-p38 signal in Tara-KD cells
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | TrioN-, GEF337-, Tiam1- and Vav2-expressing HEK293 cell lines [1] |
| Concentration: | 50 μM |
| Incubation Time: | 1 hour |
| Result: | Decreased TrioN-induced Rac activity. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 2 mg/mL ( 5.38 mM ; ultrasonic and warming and heat to 80°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.6922 mL | 13.4608 mL | 26.9215 mL |
| 5 mM | 0.5384 mL | 2.6922 mL | 5.3843 mL |
| 10 mM | --- | --- | --- |
References
- [1] Bouquier N, et al. A cell active chemical GEF inhibitor selectively targets the Trio/RhoG/Rac1 signaling pathway. Chem Biol. 2009 Jun 26;16(6):657-66.
- [2] Yano T, et al. Tara up-regulates E-cadherin transcription by binding to the Trio RhoGEF and inhibiting Rac signaling. J Cell Biol. 2011 Apr 18;193(2):319-32.