[CAS NO. 43210-67-9] Fenbendazole
PRODUCTS SPECIFICATIONS [43210-67-9]
DESCRIPTION [43210-67-9]
Overview
| MDL | MFCD00144301 |
|---|---|
| Molecular Weight | 299.35 |
| Molecular Formula | C15H13N3O2S |
| SMILES | O=C(OC)NC1=NC2=CC=C(SC3=CC=CC=C3)C=C2N1 |
3 Publications Citing Use of MCE
Summary
Fenbendazole is an orally active benzimidazole anthelmintic agent, with a broad antiparasitic range. Fenbendazole is a microtubule destabilizing agent and acts on helminthes primarily by binding to tubulin and disrupting the tubulin microtubule equilibrium. Fenbendazole stabilizes the transcriptional activator HIF-1α . Fenbendazole possesses an efficient anti-proliferative activity and induces apoptosis . Fenbendazole causes cell-cycle arrest and mitotic cell death, and has antitumor activity in mice xenografted with wild-type p53 [1] .
In Vitro
Fenbendazole (1 uM; for 24 h) significantly reduces cell growth in tumour cell lines with wild-type p53, H460 and A549 human NSCLC cell lines
[1]
.
Fenbendazole (1 uM; for 24 h) induces apoptosis and causes an increased level of p53 protein in the mitochondrial fraction
[1]
.
Fenbendazole (1 uM; for 24 h) causes cell cycle arrest in the mitotic phase in human NSCLC cells
[1]
.
Fenbendazole (1 uM; for 24 h) causes a partial alteration of the microtubule network in human non small cell lung carcinoma (NSCLC) A549 cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Cycle Analysis [1]
| Cell Line: | A549 cells |
| Concentration: | 1 uM |
| Incubation Time: | For 24 h |
| Result: |
Caused an early elevation of cyclin B1/CDK1 levels (8 h as compared to 16 h in case of control untreated cells).
p-histone H3 (Ser10) was found to be up-regulated at 12 and 24 h. |
Apoptosis Analysis [1]
| Cell Line: | A549 cells |
| Concentration: | 1 uM |
| Incubation Time: | 8, 16, 24, 32, 40, 48 h |
| Result: | The number of apoptotic cells increased in a time dependent manner with simultaneous decrease in cyclin B1 levels, and ~30% cells had undergone apoptosis after 32 h. |
Western Blot Analysis [1]
| Cell Line: | H460 cells |
| Concentration: | 1 uM |
| Incubation Time: | For 24 h |
| Result: | Caused an increased level of p53 protein in the mitochondrial fraction. |
In Vivo
Fenbendazole (1 mg; orally; every second day for 12 day) leads to a marked reduction in tumour size and weight [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Female athymic nu/nu mice were xenografted with A549 cells [1] |
| Dosage: | 1 mg/mouse |
| Administration: | Orally; every second day for 12 day |
| Result: |
Led to a marked reduction in tumour size and weight.
Led to a reduction in hemoglobin content in tumors signifying reduced tumor vascularity. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 10 mg/mL ( 33.41 mM ; Need ultrasonic)
H 2 O : 1 mg/mL ( 3.34 mM ; ultrasonic and warming and heat to 80°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.3406 mL | 16.7029 mL | 33.4057 mL |
| 5 mM | 0.6681 mL | 3.3406 mL | 6.6811 mL |
| 10 mM | 0.3341 mL | 1.6703 mL | 3.3406 mL |
References
- [1] Nilambra Dogra, et al. Fenbendazole acts as a moderate microtubule destabilizing agent and causes cancer cell death by modulating multiple cellular pathways. Sci Rep. 2018 Aug 9;8(1):11926.
- [2] Hossein Aleyasin, et al. Antihelminthic benzimidazoles are novel HIF activators that prevent oxidative neuronal death via binding to tubulin. Antioxid Redox Signal. 2015 Jan 10;22(2):121-34.
- [3] Qiwen Duan, et al. Fenbendazole as a potential anticancer drug. Anticancer Res. 2013 Feb;33(2):355-62.
Synonyms