[CAS NO. 436-77-1] Fangchinoline

Ships within

Stock

Price

Qty

Total

$0.00

Please click "REQUEST A QUOTE" button if you need other sizes or custom synthesis

request a quote

If there is no stock, or you need other sizes or custom synthesis, please:

PRODUCTS SPECIFICATIONS [436-77-1]

Catalog

HY-N1372A

Brand

MCE

CAS

436-77-1

DESCRIPTION [436-77-1]

Overview

MDL	-
Molecular Weight	608.72
Molecular Formula	C37H40N2O6
SMILES	OC1=C(OC2=CC([C@]3([H])CC(C=C4)=CC=C4O5)=C(CCN3C)C=C2OC)C([C@](N6C)([H])CC7=CC=C(OC)C5=C7)=C(CC6)C=C1OC

For research use only. We do not sell to patients.

2 Publications Citing Use of MCE

Summary

Fangchinoline is isolated from Stephania tetrandra with extensive biological activities, such as enhancing immunity, anti-inflammatory sterilization and anti-atherosclerosis. Fangchinoline, a novel HIV-1 inhibitor, inhibits HIV-1 replication by impairing gp160 proteolytic processing ^[1] . Fangchinoline targets Focal adhesion kinase (FAK) and suppresses FAK-mediated signaling pathway in tumor cells which highly expressed FAK ^[2] . Fangchinoline induces apoptosis and adaptive autophagy in bladder cancer ^[3] .

IC50 & Target

HIV-1

In Vitro

Fangchinoline (2.5-40 µM; 24-96 hours) inhibits both T24 and 5637 cells in dose-dependent manner, the IC ₅₀ values of Fangchinoline in T24 cells are 19.0 µM (24 h), 12.0 µM (48 h) and 7.57 µM (72 h), and 11.9 µM (24 h), 9.92 µM (48 h) and 7.13 µM (72 h) in 5637 cells ^[1] .
Fangchinoline (5 µM; 24 hours) induces a significant increase in the LC3-II/LC3-I ratio and a decrease in p62 in both T24 and 5637 cells, and causes a significant increase in the cleavage of caspase-3 ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay ^[3]

Cell Line:	T24 and 5637 cells
Concentration:	2.5 µM; 5 µM; 10 µM; 20 µM; 30 µM; 40 µM
Incubation Time:	24 hours; 48 hours; 96 hours
Result:	Inhibited both T24 and 5637 cells proliferation.

Western Blot Analysis ^[3]

Cell Line:	T24 and 5637 cells
Concentration:	5 µM
Incubation Time:	24 hours
Result:	Incresed LC3-II/LC3-I ratio and the cleavage of caspase-3.

Appearance

Solid

Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL ( 82.14 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6428 mL	8.2140 mL	16.4279 mL
5 mM	0.3286 mL	1.6428 mL	3.2856 mL
10 mM	0.1643 mL	0.8214 mL	1.6428 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (3.42 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.08 mg/mL (3.42 mM); Clear solution

* All of the co-solvents are available by MCE.

References

Synonyms

16H-1,24:6,9-Dietheno-11,15-metheno-2H-pyrido[2′,3′:17,18][1,11]dioxacycloeicosino[2,3,4-ij]isoquinolin-22-ol, 3,4,4a,5,16a,17,18,19-octahydro-12,21,26-trimethoxy-4,17-dimethyl-, (4aS,16aS)-

Fangchinoline

Berbaman-7-ol, 6,6′,12-trimethoxy-2,2′-dimethyl-, (1β)-

(4aS,16aS)-3,4,4a,5,16a,17,18,19-Octahydro-12,21,26-trimethoxy-4,17-dimethyl-16H-1,24:6,9-dietheno-11,15-metheno-2H-pyrido[2′,3′:17,18][1,11]dioxacycloeicosino[2,3,4-ij]isoquinolin-22-ol

(+)-Limacine

(+)-Fangchinoline

7-O-Demethyltetrandrine

16H-1,24:6,9-Dietheno-11,15-metheno-2H-pyrido[2′,3′:17,18][1,11]dioxacycloeicosino[2,3,4-ij]isoquinolin-22-ol, 3,4,4a,5,16a,17,18,19-octahydro-12,21,26-trimethoxy-4,17-dimethyl-, [4aS-(4aR*,16aR*)]-

NSC 77036

Hanfangchin B