[CAS NO. 442-51-3] Harmine
PRODUCTS SPECIFICATIONS [442-51-3]
DESCRIPTION [442-51-3]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 212.25 |
| Molecular Formula | C13H12N2O |
| SMILES | CC1=NC=CC2=C1NC3=C2C=CC(OC)=C3 |
11 Publications Citing Use of MCE
- [1]Cell Stem Cell. 2022 Apr 7;29(4):545-558.e13.
- [2]J Biomed Sci. 2022 Jun 2;29(1):34.
- [3]Cancer Biol Med. 2020 May 15;17(2):387-400.
- [4]Front Cell Dev Biol. 2022 Jan 17;9:792257.
- [5]J Cell Mol Med. 2019 Nov;23(11):7427-7437.
- [6]Prog Neuropsychopharmacol Biol Psychiatry. 2017 Jun 15;79(Pt B):258-267.
- [7]Sci Rep. 2015 Aug 3;5:12728.
- [8]Int J Oncol. 2022 Apr;60(4):45.
- [9]Onco Targets Ther. 2019 Jun 12;12:4585-4593.
- [10]Exp Ther Med. January 7, 2022.
- [11]Research Square Preprint. 2021 Aug.
Summary
IC50 & Target
|
5-HT 2A Receptor 397 nM (Ki) |
DYRK1A
|
In Vitro
Harmine inhibits tau phosphorylation by DYRK1A by selected DANDYs, with an IC 50 of 190 nM [2] .Harmine negatively regulates homologous recombination (HR) by interfering Rad51 recruitment, resulting in severe cytotoxicity in hepatoma cells. Furthermore, NHEJ inhibitor Nu7441 markedly sensitizes Hep3B cells to the anti-proliferative effects of Harmine [3] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
It is shown that brain water content is significantly increased in the TBI group. Treatment with Harmine significantly reduces the tissue water content at 1, 3 and 5 days, compared with the TBI group. Harmine treatment significantly reduces the escape latency at 3 and 5 days, compared with the TBI group. Post-TBI administration of Harmine significantly improves the motor function recovery of the rats at 1, 3 and 5 days following TBI, compared with the TBI group without Harmine treatment. The neuronal survival rate in the Harmine-treated group is significantly increased, compared with the TBI group. Administration of Harmine results in marked elevation in the expression of GLT-1, compared with the TBI group. The administration of Harmine significantly reduces the expression of caspase 3, compared with the TBI group [4] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date | Phase |
|---|---|---|---|---|
| NCT04716335 | Psychiatric University Hospital, Zurich|University of Basel |
Emotions|Mood|Cognitive Function 1, Social|Empathy
|
December 1, 2020 | Early Phase 1 |
| NCT05526430 | James Murrough|National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)|Icahn School of Medicine at Mount Sinai |
Diabetes Mellitus
|
September 13, 2022 | Phase 1 |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 30 mg/mL ( 141.34 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 4.7114 mL | 23.5571 mL | 47.1143 mL |
| 5 mM | 0.9423 mL | 4.7114 mL | 9.4229 mL |
| 10 mM | 0.4711 mL | 2.3557 mL | 4.7114 mL |
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1.
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2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution
References
- [1] Glennon RA, et al. Binding of beta-carbolines and related agents at serotonin (5-HT(2) and 5-HT(1A)), dopamine (D(2)) and benzodiazepine receptors. Drug Alcohol Depend. 2000 Aug 1;60(2):121-32.
- [2] Neumann F, et al. DYRK1A inhibition and cognitive rescue in a Down syndrome mouse model are induced by new fluoro-DANDY derivatives. Sci Rep. 2018 Feb 12;8(1):2859.
- [3] Zhang L, et al. Harmine suppresses homologous recombination repair and inhibits proliferation of hepatoma cells. Cancer Biol Ther. 2015;16(11):1585-92.
- [4] Zhong Z, et al. Treatment with harmine ameliorates functional impairment and neuronal death following traumatic brain injury. Mol Med Rep. 2015 Dec;12(6):7985-91.
Synonyms