[CAS NO. 452-06-2] 2-Aminopurine
PRODUCTS SPECIFICATIONS [452-06-2]
DESCRIPTION [452-06-2]
Overview
| MDL | MFCD00005566 |
|---|---|
| Molecular Weight | 135.13 |
| Molecular Formula | C5H5N5 |
| SMILES | NC1=NC=C2NC=NC2=N1 |
Summary
2-Aminopurine, a fluorescent analog of guanosine and adenosine, is a widely used fluorescence-decay-based probe of DNA structure. When 2-Aminopurine is inserted in anoligonucleotide, its fluorescence is highly quenched by stacking with the natural bases. 2-Aminopurine has been used to probe nucleic acid structure and dynamics [1] [2] .
In Vitro
2-Aminopurine (2AP) is not valuable as afluorescent label because its fluorescence is highly quenched by stacking with the natural bases, when it is inserted in anoligonucleotide. However, it is this very susceptibility to interbase quenching that makes 2AP an exquisitely sensitivefluorescent probe of nucleic acid structure
[1]
.
2-Aminopurine differs from adenine (6-aminopurine) only in the position of the exocyclic amine group, and yet its fluorescence intensity is one thousand times that of adenine
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 5 mg/mL ( 37.00 mM ; Need ultrasonic)
H 2 O : 1.35 mg/mL ( 9.99 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 7.4003 mL | 37.0014 mL | 74.0028 mL |
| 5 mM | 1.4801 mL | 7.4003 mL | 14.8006 mL |
| 10 mM | 0.7400 mL | 3.7001 mL | 7.4003 mL |
-
1.
Add each solvent one by one: PBS
Solubility: 2.5 mg/mL (18.50 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
References
- [1] J M Jean, et al. 2-Aminopurine fluorescence quenching and lifetimes: role of base stacking. Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):37-41.
- [2] Dehong Tan, et al. Decreased glycation and structural protection properties of γ-glutamyl-S-allyl-cysteine peptide isolated from fresh garlic scales (Allium sativum L.). Nat Prod Res. 2015;29(23):2219-22.
Synonyms