[CAS NO. 465-21-4] Bufalin

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PRODUCTS SPECIFICATIONS [465-21-4]

Catalog

HY-N0877

Brand

MCE

CAS

465-21-4

DESCRIPTION [465-21-4]

Overview

MDL	MFCD00056525
Molecular Weight	386.52
Molecular Formula	C24H34O4
SMILES	O[C@]([C@@](CC[C@@]1([H])[C@@]2(CC[C@H](O)C1)C)([H])[C@]2([H])CC3)(CC[C@@H]4C(C=C5)=COC5=O)[C@]34C

For research use only. We do not sell to patients.

5 Publications Citing Use of MCE

Summary

Bufalin is an active component isolated from Chan Su, acts as a potent Na ⁺ /K ⁺ -ATPase inhibitor, binds to the subunit α1, α2 and α3, with K _d of 42.5, 45 and 40 nM, respectively ^[1] ^[2] . Anti-cancer activity ^[2] .

IC50 & Target

Kd: 42.5 nM (Na,K-ATPase α1), 45 nM (Na,K-ATPase α2), 40 nM (Na,K-ATPase α3) ^[1]

In Vitro

Bufalin (0, 1, 2, 4 μM for 48 hours) decreases cell viability in NCI-H460 cells ^[2] .
Bufalin (2 μM) increases caspae-3, Endo G and GADD153 mRNA expression, but decreases the GRP78 mRNA expression ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay ^[2]

Cell Line:	NCI-H460 cells
Concentration:	0, 1, 2, 4 μM
Incubation Time:	48 hours
Result:	Decreased viability of NCI-H460 cells in a dose-dependent manner.

In Vivo

Bufalin (0.1, 0.2, or 0.4 mg/kg, i.p., daily for 14 days) shows significant anti-tumor activity in mice bearing NCI-H460 cells ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Forty male athymic BALB/c nu/nu mice (6-8 weeks old) ^[2]
Dosage:	0.1, 0.2, or 0.4 mg/kg
Administration:	I.P. every day until 14 days
Result:	Dose-dependently suppressed tumor growth.

Appearance

Solid

Source

toad

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL ( 258.72 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5872 mL	12.9359 mL	25.8719 mL
5 mM	0.5174 mL	2.5872 mL	5.1744 mL
10 mM	0.2587 mL	1.2936 mL	2.5872 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (6.47 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (6.47 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (5.38 mM); Clear solution

* All of the co-solvents are available by MCE.

References

Synonyms

Bufa-20,22-dienolide, 3,14-dihydroxy-, (3β,5β)-

Bufalin

5β-Bufa-20,22-dienolide, 3β,14-dihydroxy-

(3β,5β)-3,14-Dihydroxybufa-20,22-dienolide

NSC 89595

B 0261