[CAS NO. 475231-21-1] 3'-Hydroxypterostilbene
PRODUCTS SPECIFICATIONS [475231-21-1]
DESCRIPTION [475231-21-1]
Overview
| MDL | MFCD12407156 |
|---|---|
| Molecular Weight | 272.30 |
| Molecular Formula | C16H16O4 |
| SMILES | COC1=CC(/C=C/C2=CC=C(O)C(O)=C2)=CC(OC)=C1 |
1 Publications Citing Use of MCE
Summary
3'-Hydroxypterostilbene is a Pterostilbene (HY-N0828) analogue. 3'-Hydroxypterostilbene inhibits the growth of COLO 205, HCT-116 and HT-29 cells with IC 50 s of 9.0, 40.2 and 70.9 µM, respectively. 3'-Hydroxypterostilbene significantly down-regulates PI3K/Akt and MAPKs signaling pathways and effectively inhibits the growth of human colon cancer cells by inducing apoptosis and autophagy . 3'-Hydroxypterostilbene can be used for the research of cancer [1] .
IC50 & Target
IC50: 9.0 μM (COLO 205),40.2 μM (HCT-116), 70.9 μM (HT-29) [1]
In Vitro
3'-Hydroxypterostilbene (5-50 μM; 24 h) affects cell proliferation of COLO 205, HCT-116 and HT-29
[1]
.
3'-Hydroxypterostilbene (5-100 μM; 24 h) induces cell apoptosis and the concentration of ROS plays an important role as an early mediator in 3'-Hydroxypterostilbene-induces apoptosis
[1]
.
3'-Hydroxypterostilbene (5-50 μM; 24 h) induces caspase-9 cleavage, increases caspase-3 activity and inhibits the mTOR/p70S6K, PI3K/Akt and MAPKs signaling pathways in COLO 205 cells
[1]
.
3'-Hydroxypterostilbene (25-50 μM; 24 h) induces autophagy in COLO 205 cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [1]
| Cell Line: | COLO 205, HCT-116 and HT-29 cell lines |
| Concentration: | 5, 10, 25 and 50 μM |
| Incubation Time: | 24 hours |
| Result: | Inhibited cell proliferation of COLO 205, HCT-116 and HT-29 cells with IC 50 s of 9.0, 40.2 and 70.9 μM, respectively. |
Apoptosis Analysis [1]
| Cell Line: | COLO 205 cells |
| Concentration: | 5-100 μM |
| Incubation Time: | 24 hours |
| Result: | Induced apoptosis in human colorectal carcinoma cells. |
Western Blot Analysis [1]
| Cell Line: | COLO 205 cells |
| Concentration: | 5-50 μM |
| Incubation Time: | 24 hours |
| Result: | Degraded 116 kDa PARP into 85 kDa fragments and induced DFF-45 protein degradation and increased the amounts of LC3B I/II proteins than pterostilbene. Markedly decreased phosphorylation of mTOR, p70S6K (Thr389), PI3K, Akt and p38 MAPK in cells. |
Cell Autophagy Assay [1]
| Cell Line: | COLO 205 cells |
| Concentration: | 25 and 50 μM |
| Incubation Time: | 24 hours |
| Result: | Showed an inducing effect on autophagy than pterostilbene in COLO 205 cells. |
In Vivo
3'-Hydroxypterostilbene (10 mg/kg; i.p. once per day for 15 days) strongly inhibits tumor growth in vivo [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | 3-4 week old BALB/c nude mice with human colorectal carcinoma COLO 205 tumor xenografts [1] |
| Dosage: | 10 mg/kg |
| Administration: | Intraperitoneal injection; 10 mg/kg once per day for 15 days |
| Result: | Significantly inhibited tumor volume and tumor weight after 15 days treatment compared with the pterostilbene-treated mice, and decreased the protein levels of COX-2, MMP-9, VEGF, cyclin D1 and pro-caspase-3 in mice. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 125 mg/mL ( 459.05 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.6724 mL | 18.3621 mL | 36.7242 mL |
| 5 mM | 0.7345 mL | 3.6724 mL | 7.3448 mL |
| 10 mM | 0.3672 mL | 1.8362 mL | 3.6724 mL |
Synonyms