[CAS NO. 478-01-3] Nobiletin
PRODUCTS SPECIFICATIONS [478-01-3]
DESCRIPTION [478-01-3]
Overview
| MDL | MFCD03273560 |
|---|---|
| Molecular Weight | 402.39 |
| Molecular Formula | C21H22O8 |
| SMILES | O=C1C=C(C2=CC=C(OC)C(OC)=C2)OC3=C(OC)C(OC)=C(OC)C(OC)=C13 |
9 Publications Citing Use of MCE
- [1]Acta Pharm Sin B. 2021 Jan;11(1):143-155.
- [2]Int J Biol Sci. 2022 Sep 11;18(15):5698-5712.
- [3]J Agric Food Chem. 2022 Feb 9;70(5):1536-1546.
- [4]Sci Rep. 2021 Jun 3;11(1):11796.
- [5]Biol Reprod. 2021 Oct 7;ioab184.
- [6]Reprod Fert Develop. 2021, 33(2): 135-135.
- [7]Reproduction, Fertility and Development. 2019, 32, 164-165.
- [8]Reprod Fert Develop. 2018, 31(1): 212-212.
- [9]E3S Web of Conferences. 233, 02010 (2021).
Summary
Nobiletin is a poly-methoxylated flavone from the citrus peel that improves memory loss. Nobiletin is a retinoid acid receptor-related orphan receptors (RORs) agonist. Nobiletin can reduce reactive oxygen species (ROS) levels in differentiated C2C12 myotubes and has anti-inflammation and anti-cancer properties, including anti-angiogenesis, anti-proliferation, anti-metastasis and induced apoptosis [1] [2] [3] [4] .
IC50 & Target
In Vitro
Nobiletin (0-100 μM; 24 hours; U2OS and HOS cells) treatment progressively reduces protein expressions of MMP-2 and MMP-9. In U2OS and HOS cells, Nobiletin considerably reduces the phosphorylation of p-IKKα/β and p-IκBα, and protein expression of NF-κB in the cell nuclear fraction with the concomitant increase of the NF-κB expression in the cytosolic fraction. Nobiletin down-regulates the p-CREB and the SP-1 expressions in the nuclear fraction, whereas Nobiletin does not affect c-Jun and c-Fos expressions
[1]
.
Nobiletin (0-100 μM; 24 hours; U2OS and HOS cells) treatment significantly reduces mRNA expressions of MMP-2 and MMP-9 dose-dependently in U2OS and HOS cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | U2OS and HOS cells |
| Concentration: | 0 μM, 25 μM, 50 μM, 75 μM, 100 μM |
| Incubation Time: | 24 hours |
| Result: | Progressively reduced protein expressions of MMP-2 and MMP-9. Considerably reduced the phosphorylation of p-IKKα/β and p-IκBα, and protein expression of NF-κB in the cell nuclear fraction with the concomitant increase of the NF-κB expression in the cytosolic fraction. Down-regulated the p-CREB and the SP-1 expressions in the nuclear fraction in U2OS and HOS cells. |
RT-PCR [1]
| Cell Line: | U2OS and HOS cells |
| Concentration: | 0 μM, 25 μM, 50 μM, 75 μM, 100 μM |
| Incubation Time: | 24 hours |
| Result: | Significantly reduced mRNA expressions of MMP-2 and MMP-9 dose-dependently in U2OS and HOS cells. |
In Vivo
Nobiletin (0.1% of regular diet; oral administration; daily; for 20 weeks; male C57BL/6 mice) treatment restores glucose homeostasis and promots energy expenditure and circadian activity in aged mice [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | 20- to 22-month-old male C57BL/6 mice [2] |
| Dosage: | 0.1% of regular diet |
| Administration: | Oral administration; daily; for 20 weeks |
| Result: | Fully restored glucose tolerance in aged mice, and increased basal body temperature and cold tolerance in aged mice. Led to a twofold increase in distance run/day on voluntary wheels compared with aged regular diet-fed mice during the active phase. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 50 mg/mL ( 124.26 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.4852 mL | 12.4258 mL | 24.8515 mL |
| 5 mM | 0.4970 mL | 2.4852 mL | 4.9703 mL |
| 10 mM | 0.2485 mL | 1.2426 mL | 2.4852 mL |
References
- [1] Cheng HL, et al. Nobiletin inhibits human osteosarcoma cells metastasis by blocking ERK and JNK-mediated MMPs expression. Oncotarget. 2016 Jun 7;7(23):35208-23.
- [2] Nohara K, et al. Nobiletin fortifies mitochondrial respiration in skeletal muscle to promote healthy aging against metabolic challenge. Nat Commun. 2019 Aug 28;10(1):3923.
- [3] Takito J, et al. Nerve growth factor enhances the CRE-dependent transcriptional activity activated by nobiletin in PC12 cells. Can J Physiol Pharmacol. 2016 Jul;94(7):728-33.
- [4] He B, et al. The Small Molecule Nobiletin Targets the Molecular Oscillator to Enhance Circadian Rhythms and Protect against Metabolic Syndrome. Cell Metab. 2016 Apr 12;23(4):610-21.
Synonyms