[CAS NO. 514-47-6] Euphol
PRODUCTS SPECIFICATIONS [514-47-6]
DESCRIPTION [514-47-6]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 426.72 |
| Molecular Formula | C30H50O |
| SMILES | C[C@@]1(CC2)[C@@](CC[C@]1([C@H](C)CC/C=C(C)/C)[H])(C)C3=C2[C@]4(C)[C@](CC3)([H])C(C)(C)[C@@H](O)CC4 |
Summary
Euphol is a tetracyclic triterpene alcohol isolated from the sap of Euphorbia tirucalli with anti-mutagenic, anti-inflammatory and immunomodulatory effects, orally active. Euphol inhibits the monoacylglycerol lipase (MGL) activity via a reversible mechanism ( IC 50 =315 nM). MGL inhibition in the periphery modulates the endocannabinoid system to block the development of inflammatory pain [1] .
IC50 & Target
IC50: 315 nM (monoacylglycerol lipase activity) [1]
In Vitro
Euphol (0.01-0.3 mM; 24-72 hours) markedly inhibits T47D cells proliferation, the IC
50
values of euphol treatment for 24, 48 and 72 h were 0.26, 0.22 and 0.13 mM, respectively
[2]
.
Euphol (0.03 mM; 48 or 72 hours) leads to cell cycle arrest by regulating expression of cell cycle-associated proteins
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [2]
| Cell Line: | T47D cells |
| Concentration: | 0.01, 0.03, 0.1 and 0.3 mM |
| Incubation Time: | 24, 48 and 72 hours |
| Result: | Decreased the percentage of viable cells. |
Western Blot Analysis [2]
| Cell Line: | T47D cells |
| Concentration: | 0.03 mM |
| Incubation Time: | 48 and 72 hours |
| Result: | Increased the expression of p21 and p27, reduced the expression of cyclin A, B1 and D. |
Appearance
Solid
Source
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 25 mg/mL ( 58.59 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.3435 mL | 11.7173 mL | 23.4346 mL |
| 5 mM | 0.4687 mL | 2.3435 mL | 4.6869 mL |
| 10 mM | 0.2343 mL | 1.1717 mL | 2.3435 mL |
References
- [1] Dutra RC, et al. Euphol, a tetracyclic triterpene produces antinociceptive effects in inflammatory and neuropathic pain: the involvement of cannabinoid system. Neuropharmacology. 2012 Sep;63(4):593-605.
- [2] Wang L, et al. Euphol arrests breast cancer cells at the G1 phase through the modulation of cyclin D1, p21 and p27 expression. Mol Med Rep. 2013 Oct;8(4):1279-85.
Synonyms