[CAS NO. 535-96-6] Marein
PRODUCTS SPECIFICATIONS [535-96-6]
DESCRIPTION [535-96-6]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 450.39 |
| Molecular Formula | C21H22O11 |
| SMILES | O=C(C1=CC=C(O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)C(O)=C1O)/C=C/C3=CC=C(O)C(O)=C3 |
Summary
Marein has the neuroprotective effect due to a reduction of damage to mitochondria function and activation of the AMPK signal pathway . Marein improves insulin resistance induced by high glucose in HepG2 cells through CaMKK/AMPK/GLUT1 to promote glucose uptake, through IRS/Akt/GSK-3β to increase glycogen synthesis, and through Akt/FoxO1 to decrease gluconeogenesis. Marein is a HDAC inhibitor with an IC 50 of 100 µM. Marein has beneficial antioxidative, antihypertensive, antihyperlipidemic and antidiabetic effects [1] [2] [3] .
IC50 & Target
IC50: 100 µM (HDAC) [3]
In Vitro
Marein (0-1000 µM; 24 h) inhibits HDAC activity and TNFα-induced NF-κB activation with IC
50
values of 100 and ＞200 μM, respectively
[1]
.
Marein (1.25-40 μM; 24 h) promotes glucose uptake in HepG2 cells
[2]
.
Marein (5-10 μM; 24 h) promotes GLUT1 translocation from intercellular vesicles to the plasma membrane, increases hepatic glycogen content and down-regulates expression levels of G6Pase and PEPCK in HepG2 cells
[2]
.
Marein (5-10 μM; 24 h) stimulates 2-NBDG uptake, and it can be reduced by STO-609 and compound C which is a inhibitor of AMPK
[2]
.
Marein (0-40 μM; 24 h) affects the cytotoxicity of MG in PC12 cells
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [2]
| Cell Line: | HepG2 cell line |
| Concentration: | 5 and 10 μM |
| Incubation Time: | 24 hours |
| Result: | Increased GLUT1 translocation to plasma membrane and decreased the phosphorylation level of AS160 and GSK-3β in HepG2 cells. |
Western Blot Analysis [2]
| Cell Line: | HepG2 cell line |
| Concentration: | 10 μM |
| Incubation Time: | 0-10 hours |
| Result: | Time- and dose-dependently induced phosphorylation of AMPK and Akt in HepG2 cells. |
Cell Viability Assay [3]
| Cell Line: | PC12 cell line |
| Concentration: | 0-40 μM |
| Incubation Time: | 24 hours |
| Result: | Prevened MG-induced loss of viability in PC12 cells. |
Apoptosis Analysis [3]
| Cell Line: | PC12 cell line |
| Concentration: | 5-10 μM |
| Incubation Time: | 30 min |
| Result: | Protected PC12 cells from MG-induced apoptosis. |
Western Blot Analysis [3]
| Cell Line: | PC12 cell line |
| Concentration: | 5-10 μM |
| Incubation Time: | 30 min |
| Result: | Increased phospho-AMPKα (Thr172) in PC12 cells. |
Appearance
Solid
Source
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 25 mg/mL ( 55.51 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.2203 mL | 11.1015 mL | 22.2030 mL |
| 5 mM | 0.4441 mL | 2.2203 mL | 4.4406 mL |
| 10 mM | 0.2220 mL | 1.1101 mL | 2.2203 mL |
References
- [1] B Orlikova, et al. Natural chalcones as dual inhibitors of HDACs and NF-κB. Oncol Rep. 2012 Sep;28(3):797-805.
- [2] Baoping Jiang, et al. Protective effects of marein on high glucose-induced glucose metabolic disorder in HepG2 cells. Phytomedicine. 2016 Aug 15;23(9):891-900.
- [3] Baoping Jiang, et al. Marein protects against methylglyoxal-induced apoptosis by activating the AMPK pathway in PC12 cells. Free Radic Res. 2016;50(11):1173-1187.
Synonyms