[CAS NO. 58131-57-0] NSC-207895
PRODUCTS SPECIFICATIONS [58131-57-0]
DESCRIPTION [58131-57-0]
Overview
| MDL | MFCD01660065 |
|---|---|
| Molecular Weight | 279.25 |
| Molecular Formula | C11H13N5O4 |
| SMILES | O=[N+](C1=CC=C(N2CCN(C)CC2)C3=[N+]([O-])ON=C31)[O-] |
Summary
In Vitro
NSC-207895 (0.5 μM, 30 min), a MDM2 inhibitor, upregulates p53 protein levels
[2]
.
NSC-207895, MDMX inhibitor, downregulates the expression of MDMX in AML cells at the mRNA and protein levels
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [2]
| Cell Line: | RAW 264.7 cells. |
| Concentration: | 0.5 μM. |
| Incubation Time: | 30 min (stimulated with LPS (100 ng/ml) for 6 h). |
| Result: | Upregulated p53 protein levels. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 31.25 mg/mL ( 111.91 mM ; ultrasonic and warming and heat to 60°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.5810 mL | 17.9051 mL | 35.8102 mL |
| 5 mM | 0.7162 mL | 3.5810 mL | 7.1620 mL |
| 10 mM | 0.3581 mL | 1.7905 mL | 3.5810 mL |
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1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: 2.08 mg/mL (7.45 mM); Suspended solution; Need ultrasonic
References
- [1] Laura Kapitzky, et al. Cross-species chemogenomic profiling reveals evolutionarily conserved drug mode of action. Mol Syst Biol. 2010 Dec 21;6:451.
- [2] Erdenezaya Odkhuu, et al. Lipopolysaccharide downregulates the expression of p53 through activation of MDM2 and enhances activation of nuclear factor-kappa B. Immunobiology. 2015 Jan;220(1):136-41.
- [3] Luis A Carvajal, et al. Dual inhibition of MDMX and MDM2 as a therapeutic strategy in leukemia. Sci Transl Med. 2018 Apr 11;10(436):eaao3003.
Synonyms