[CAS NO. 646530-37-2] UTL-5g
PRODUCTS SPECIFICATIONS [646530-37-2]
DESCRIPTION [646530-37-2]
Overview
| MDL | MFCD08724989 |
|---|---|
| Molecular Weight | 271.10 |
| Molecular Formula | C11H8Cl2N2O2 |
| SMILES | O=C(C1=NOC(C)=C1)NC2=CC=C(Cl)C=C2Cl |
Summary
In Vitro
RAW 264.7 macrophages are transfected with the respective reporter assay plasmids, pretreated with UTL-5g at 1, 10 or 50 µM for 60 min and then challenged with 100 ng/ml LPS. After a 16 h incubation, transcription factor activity is measured. Transcription factors that shows a UTL-5g dose-dependent decrease in activity in two experiments are categorized as being disrupted by UTL-5g.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
UTL-5g (GBL-5g) lowers levels of TGF-β and TNF-α elevated by lung irradiation
[1]
.
UTL-5g (60 mg/kg; p.o.; daily for 4 days) shows positive effects in increasing the survival rates and extending the survival times
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | C57BL/6, male mice (8-10 weeks) [1] |
| Dosage: | 15, 30, and 60 mg/kg |
| Administration: | I.p.; before irradiation, daily x 5 |
| Result: | Blood levels of TGF-β were lowered. |
| Animal Model: | BDF1 female mice [3] |
| Dosage: | P.o.; daily for 4 days |
| Administration: | 60 mg/kg (30 min before i.p. injection of Cisplatin at 10, 15, and 20 mg/kg respectively on Day 0) |
| Result: | Increased the survival rate and delayed the time to death for mice treated with 150% of the maximum tolerated dose (MTD) of Cisplatin (15 mg/kg). At 200% of the MTD of Cisplatin (20 mg/kg), treatment of UTL-5g increased the survival rate and delayed the time to death. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 100 mg/mL ( 368.87 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.6887 mL | 18.4434 mL | 36.8868 mL |
| 5 mM | 0.7377 mL | 3.6887 mL | 7.3774 mL |
| 10 mM | 0.3689 mL | 1.8443 mL | 3.6887 mL |
-
1.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution
References
- [1] Stephen Brown, et al. UTL-5g Lowers Levels of TGF-β and TNF-α Elevated by Lung Irradiation
- [2] Carruthers NJ, et al. Phosphoproteome and transcription factor activity profiling identify actions of the anti-inflammatory agent UTL-5g in LPS stimulated RAW 264.7 cells including disrupting actin remodeling and STAT-3 activation. Eur J Pharmacol. 2017;8
- [3] Shaw J, et al. The small-molecule TNF-α inhibitor, UTL-5g, delays deaths and increases survival rates for mice treated with high doses of cisplatin. Cancer Chemother Pharmacol. 2013;72(3):703-707.
Synonyms