[CAS NO. 64761-48-4] Harringtonolide
PRODUCTS SPECIFICATIONS [64761-48-4]
DESCRIPTION [64761-48-4]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 310.34 |
| Molecular Formula | C19H18O4 |
| SMILES | O=C1C=C(CC2)C3=C([C@H]4C(C5O6)C3[C@@]2([C@H](C)[C@H]5O4)C6=O)C(C)=C1 |
Summary
Harringtonolide is a potent RACK1 inhibitor ( IC 50 =39.66 μM in A375 cells). Harringtonolide inhibits the epithelial-mesenchymal transition (EMT) process and cell proliferation by affecting the interaction between FAK and RACK1 . Harringtonolide has plant growth inhibitory, antiviral, anti-inflammatory, and antiproliferation activities [1] .
IC50 & Target
IC 50 : 39.66 μM (RACK1) in A375 cells [1]
In Vitro
Harringtonolide (0-50 μM; 24 hours) exhibits good antiproliferation activity in A375 cells with IC
50
of 39.66 μM
[1]
.
Harringtonolide (0-20 μM; 1 hour) restrains the proteolysis of RACK1 by Pronase, and protects temperature-dependent degradation of RACK1
[1]
.
Harringtonolide (0-4 μM; 24 hours) suppresses the phosphorylation of FAK dose-dependently and inhibits Src and STAT3, the downstream proteins of FAK
[1]
.
Harringtonolide (0-4 μM; 24 hours) dose-dependently inhibits the RACK1–FAK interaction in A375 cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay
| Cell Line: | A375 melanoma cells [1] |
| Concentration: | 0-50 μM |
| Incubation Time: | 24 hours |
| Result: | Showed good antiproliferation activity with IC 50 of 39.66 μM. |
Western Blot Analysis
| Cell Line: | A375 melanoma cells [1] |
| Concentration: | 0, 0.5, 1, 2, 4 μM |
| Incubation Time: | 24 hours |
| Result: | Suppressed the phosphorylation of FAK dose-dependently and inhibited Src and STAT3, the downstream proteins of FAK. |
Appearance
Solid
Source
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)