[CAS NO. 65893-94-9] GlabrescioneB

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PRODUCTS SPECIFICATIONS [65893-94-9]

Catalog

HY-122590

Brand

MCE

CAS

65893-94-9

DESCRIPTION [65893-94-9]

Overview

MDL	-
Molecular Weight	450.52
Molecular Formula	C27H30O6
SMILES	O=C1C(C2=CC=C(OC/C=C(C)/C)C(OC/C=C(C)/C)=C2)=COC3=CC(OC)=CC(OC)=C13

For research use only. We do not sell to patients.

Summary

Glabrescione B is the first compound that binds the Hedgehog (Hh) modulator Gli1 . Glabrescione B impairs its activity by interfering with Gli1-DNA interaction. Glabrescione B inhibits the growth of Hedgehog-dependent tumor cells, the self-renewal ability, and clonogenicity of tumor-derived stem cells ^[1] ^[2] .

IC50 & Target

Gli1-DNA Interaction ^[1]

In Vitro

Glabrescione B (5 μM; 24-72 hours) inhibits the growth of Gli-dependent basal cell carcinoma ^[2] .
Glabrescione B (1-10 μM; 24-48 hours) decreases Gli1 mRNA expression levels ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay ^[1]

Cell Line:	ASZ001 BCC cells
Concentration:	5 μM
Incubation Time:	24-72 hours
Result:	Basal cell carcinoma cell proliferation was impaired.

Western Blot Analysis ^[1]

Cell Line:	ASZ001 BCC cells
Concentration:	1-10 μM
Incubation Time:	24-48 hours
Result:	Gli1 mRNA expression levels was decreased.

In Vivo

Animal Model:	Female NOD/SCID mice (ASZ001 BCC allografts) ^[2]
Dosage:	100 μmol/kg
Administration:	I.p.; every second day for 18 days
Result:	A significant reduction of tumor growth as well as Gli1 mRNA levels was observed.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL ( 221.97 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2197 mL	11.0983 mL	22.1966 mL
5 mM	0.4439 mL	2.2197 mL	4.4393 mL
10 mM	0.2220 mL	1.1098 mL	2.2197 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: 2.5 mg/mL (5.55 mM); Suspended solution; Need ultrasonic
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: 2.5 mg/mL (5.55 mM); Suspended solution; Need ultrasonic
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (5.55 mM); Clear solution

* All of the co-solvents are available by MCE.