[CAS NO. 67953-08-6] GPNA hydrochloride
PRODUCTS SPECIFICATIONS [67953-08-6]
DESCRIPTION [67953-08-6]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 303.70 |
| Molecular Formula | C11H14ClN3O5 |
| SMILES | OC([C@@H](N)CCC(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O.[H]Cl |
3 Publications Citing Use of MCE
Summary
GPNA hydrochloride is a well known substrate of the enzyme γ-glutamyltransferase ( GGT ). GPNA hydrochloride is a specific glutamine (Gln) transporter ASCT2 inhibitor. GPNA hydrochloride also inhibit Na + -dependent carriers, such as SNAT family (SNAT1/2/4/5), and the Na + -independent leucine transporters LAT1/2. GPNA reversibly induces apoptosis in A549 cells [1] .
IC50 & Target
ASCT2 [1]
In Vitro
GPNA (10-1000 μM; 48 hours) induces a dose-dependent decrease of cell viability in A549 cells [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | A549 cells |
| Concentration: | 10, 100, 250, 500 , and 1000 μM |
| Incubation Time: | 48 hours |
| Result: | Induced a dose-dependent decrease of cell viability, with an IC50 roughly corresponding to ~250 μM. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 125 mg/mL ( 411.59 mM ; Need ultrasonic)
H 2 O : 2 mg/mL ( 6.59 mM ; ultrasonic and warming and heat to 60°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.2927 mL | 16.4636 mL | 32.9272 mL |
| 5 mM | 0.6585 mL | 3.2927 mL | 6.5854 mL |
| 10 mM | 0.3293 mL | 1.6464 mL | 3.2927 mL |
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1.
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2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (6.85 mM); Clear solution
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3.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.08 mg/mL (6.85 mM); Clear solution
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4.
Add each solvent one by one: PBS
Solubility: 1 mg/mL (3.29 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Synonyms