[CAS NO. 697235-38-4] Silvestrol

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PRODUCTS SPECIFICATIONS [697235-38-4]

Catalog

HY-13251

Brand

MCE

CAS

697235-38-4

DESCRIPTION [697235-38-4]

Overview

MDL	-
Molecular Weight	654.66
Molecular Formula	C34H38O13
SMILES	O=C([C@H]([C@H]1C2=CC=CC=C2)[C@@H](O)[C@]3(O)[C@@]1(C4=CC=C(OC)C=C4)OC5=CC(O[C@@H]6O[C@@H]([C@H](O)CO)CO[C@H]6OC)=CC(OC)=C35)OC

For research use only. We do not sell to patients.

40 Publications Citing Use of MCE

Summary

Silvestrol is a eukaryotic translation initiation factor 4A ( eIF4A ) inhibitor isolated from Agave americana Linn. . Silvestrol induces autophagy and caspase-mediated apoptosis ^[1] ^[2] ^[3] .

IC50 & Target

eIF4A ^[1]

In Vitro

Silvestrol is a specific eIF4A-targeting translation inhibitor. Silvestrol exhibits significant cytotoxic activity against many human cancer cell lines, such as lung, prostate, and breast cancer with IC ₅₀ values ranging from 1 to 7 nM ^[1] .
Silvestrol significantly reduces the number of LNCaP cell colonies. Silvestrol (30 nM, 120 nM) induces apoptosis in LNCaP cells, through the mitochondrial pathway. Apaf-1, Caspase-2, caspase-9, and caspase-10 are involved in Silvestrol-induced apoptosis but caspase-3 and 7 are not ^[2] .
Silvestrol induces caspase-3 activation and apoptotic cell death in a time- and dose-dependent manner. Silvestrol-mediated cell death is attenuated in ATG7-null mouse embryonic fibroblasts (MEFs) lacking a functional autophagy protein ^[3] .
Silvestrol (50 nM) exerts an immediate inhibitory effect and causes near-static cell index compared with the control cells. Silvestrol (6.25 nM) enhances proliferation more than the vehicle control-treated cells, whereas a higher concentration of Silvestrol (50 nM) can inhibit cell proliferation. Silvestrol and episilvestrol display synergistic effects in combination with CDDP ^[4] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Silvestrol (1.5 mg/kg, i.p.) does not adversely affect production of human IgG by xenografted B-lymphocytes in mice. Silvestrol significantly prolongs survival compared to vehicle. There is no such lymphocyte infiltration detected in the spleens of any of the Silvestrol-treated mice, and nor do these animals exhibit any other obvious signs of lymphoma upon necropsy ^[5] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 6.6 mg/mL ( 10.08 mM )

H ₂ O : < 0.1 mg/mL (insoluble)

* "≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5275 mL	7.6376 mL	15.2751 mL
5 mM	0.3055 mL	1.5275 mL	3.0550 mL
10 mM	0.1528 mL	0.7638 mL	1.5275 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Silvestrol is prepared in 5.2% Tween 80 5.2% PEG 400 ^[6] .
2.

Silvestrol is dissolved in 20%(w/v) 2-hydroxyproply beta-cyclodextrin vehicle at a concentration of 125 μg/mL and injected into mice i.p. ^[7] .
3.

Silvestrol is formulated in 30% 2-hydroxypropyl-β-cyclodextrin ^[8] .

References

Synonyms

1H-Cyclopenta[b]benzofuran-2-carboxylic acid, 6-[[(2S,3R,6R)-6-[(1R)-1,2-dihydroxyethyl]-3-methoxy-1,4-dioxan-2-yl]oxy]-2,3,3a,8b-tetrahydro-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-, methyl ester, (1R,2R,3S,3aR,8bS)-

Silvestrol

(-)-Silvestrol