[CAS NO. 7240-37-1] 7-Aminoactinomycin D
PRODUCTS SPECIFICATIONS [7240-37-1]
DESCRIPTION [7240-37-1]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 1270.43 |
| Molecular Formula | C62H87N13O16 |
| SMILES | O=C(N[C@H](C(N1[C@@](C(N(C)CC(N(C)[C@@H](C(C)C)C(O[C@@H]2C)=O)=O)=O)([H])CCC1)=O)C(C)C)[C@H]2NC(C(C(C(OC3=C(C)C(N)=C4)=C5C)=NC3=C4C(N[C@@H]6C(N[C@H](C(N7[C@@](C(N(C)CC(N(C)[C@@H](C(C)C)C(O[C@@H]6C)=O)=O)=O)([H])CCC7)=O)C(C)C)=O)=O)=C(N)C5=O)=O |
2 Publications Citing Use of MCE
Summary
In Vitro
7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. 7-Aminoactinomycin D staining with 5 μg/mL, 10 μg/mL, and 20 μg/mL, but not with 1 μg/mL, is suitable for quantification of apoptosis in flow cytometry
[1]
.
7-Aminoactinomycin D is frequently used to stain and exclude dead cells in flow cytometry at low concentrations (0.5-5 μg/mL). At higher concentrations (10-20 μg/mL), 7-Aminoactinomycin D has also been used to distinguish between viable cells (7-AAD
negative
) and apoptotic cells (7-AAD
dim
) or dead cells (7-AAD
bright
) using the fact that permeability of the cell membrane, and hence fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, protect from light
* The compound is unstable in solutions, freshly prepared is recommended.
Solvent & Solubility
DMSO : 100 mg/mL ( 78.71 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 0.7871 mL | 3.9357 mL | 7.8714 mL |
| 5 mM | 0.1574 mL | 0.7871 mL | 1.5743 mL |
| 10 mM | 0.0787 mL | 0.3936 mL | 0.7871 mL |
References
- [1] Wadkins RM, et al. Actinomycin D and 7-aminoactinomycin D binding to single-stranded DNA. Biochemistry. 1991 Oct 1;30(39):9469-78.
- [2] Nadine C L Zembruski, et al. 7-Aminoactinomycin D for apoptosis staining in flow cytometry. Anal Biochem. 2012 Oct 1;429(1):79-81.
- [3] Hanen Sellami, et al. Molecular detection of Chlamydia trachomatis and other sexually transmitted bacteria in semen of male partners of infertile couples in Tunisia: the effect on semen parameters and spermatozoa apoptosis markers. PLoS One. 2014 Jul 14;9(7):e98903.