[CAS NO. 80286-36-8] Balanophonin
PRODUCTS SPECIFICATIONS [80286-36-8]
DESCRIPTION [80286-36-8]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 356.37 |
| Molecular Formula | C20H20O6 |
| SMILES | OC[C@@H]1C2=CC(/C=C/C=O)=CC(OC)=C2O[C@H]1C3=CC(OC)=C(C=C3)O |
Summary
IC50 & Target
|
COX-2 |
p38 |
In Vitro
Balanophonin (1-10 μM; 24 h) reduces Lipopolysaccharides (LPS; HY-D1056)-mediated TLR4 activation and NO production in BV2 cells
[1]
.
Balanophonin (1-10 μM; 6 h) reduces iNOS and COX2 protein expression and TNF-α and IL-1β production in LPS-induced microglial cells
[1]
.
Balanophonin (1-10 μM; 30 min) effectively inhibits MAPK activation
[1]
.
Balanophonin (1-10 μM; 24 h) inhibits neuronal cell death resulting from LPS-activated microglia by regulating cleaved caspase-3 and poly ADP ribose polymerase (PARP) cleavage in N2a cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | LPS-activated BV-2 cells |
| Concentration: | 1, 5, and 10 μM |
| Incubation Time: | 30 min for MAPKs, 6 h for iNOS and COX2 and 24 h for PGE2, TNF-α and IL-1β |
| Result: | Downregulated TLR4. Reduced iNOS and COX2 expression. Significantly reduced the secretion of TNF-α and IL-1β. Decreased the phosphorylation of MAPKs such as pERK, pJNK, and p-p38. |
Western Blot Analysis [1]
| Cell Line: | Neuroblastoma N2a cells |
| Concentration: | 1, 5, and 10 μM |
| Incubation Time: | 24 h |
| Result: | Slightly increased Bcl-2. Inhibited caspase-3 activation and PARP cleavage. |
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.