[CAS NO. 900305-37-5] PFI-4

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PRODUCTS SPECIFICATIONS [900305-37-5]

Catalog

HY-18664

Brand

MCE

CAS

900305-37-5

DESCRIPTION [900305-37-5]

Overview

MDL	MFCD14744412
Molecular Weight	380.44
Molecular Formula	C21H24N4O3
SMILES	O=C(NC1=C(N2CCCC2)C=C(N3C)C(N(C)C3=O)=C1)C4=CC=CC=C4OC

For research use only. We do not sell to patients.

Summary

PFI-4 (compound 11) is a potent and highly selective BRPF1 Bromodomain (BRPF1B) inhibitor, with an IC ₅₀ of 172 nM. PFI-4 can be used to explore the functional mechanisms of the HBO1/BRPF1 complex and to study bone loss and osteolytic malignant bone lesions ^[1] ^[2] .

IC50 & Target

IC50: 3.517 μM (BRPF2), >10 μM (BRD4 (1)), 0.172 μM (BRPF1B), >10 μM (TRIM24) ^[1] .

In Vitro

PFI-4 (1.25 µM; 7 or 11 days) inhibits differentiation of human osteoclasts ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay ^[1]

Cell Line:	Osteoclasts
Concentration:	1.25 µM
Incubation Time:	7 or 11 days
Result:	Significantly reduces MMP9 secretion in osteoclasts.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 26 mg/mL ( 68.34 mM ; Need ultrasonic and warming)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6285 mL	13.1427 mL	26.2854 mL
5 mM	0.5257 mL	2.6285 mL	5.2571 mL
10 mM	0.2629 mL	1.3143 mL	2.6285 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 1 mg/mL (2.63 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 1 mg/mL (2.63 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 1 mg/mL (2.63 mM); Clear solution

* All of the co-solvents are available by MCE.