[CAS NO. 924473-59-6] T56-LIMKi
PRODUCTS SPECIFICATIONS [924473-59-6]
DESCRIPTION [924473-59-6]
Overview
| MDL | MFCD08495674 |
|---|---|
| Molecular Weight | 389.33 |
| Molecular Formula | C19H14F3N3O3 |
| SMILES | O=C(C1=CC(C)=NO1)NC2=CC=CC(C(NC3=CC=CC(C(F)(F)F)=C3)=O)=C2 |
1 Publications Citing Use of MCE
Summary
T56-LIMKi is a selective inhibitor of LIMK2 ; inhibits the growth of Panc-1 cells with an IC 50 of 35.2 μM.
IC50 & Target
|
LIMK2 |
In Vitro
T56-LIMKi efficiently inhibits the growth of ST88-14, U87, Panc-1 cells, A549 lung cancer cells with IC 50 values of 18.3, 7.4, 35.2 and 90 μM, respectively. T56-LIMKi decreases phosphorylated cofilin (p-cofilin) levels and thus inhibits growth of several cancerous cell lines, including those of pancreatic cancer, glioma and schwannoma [1] . It blocks the phosphorylation of cofilin which leads to actin severance and inhibition of tumor cell migration, tumor cell growth, and anchorage-independent colony formation in soft agar. T56-LIMKi (10-50 μM) reduces p-cofilin in a dose-dependent manner in NF1 −/− MEFs with an IC 50 of 30 μM. Notably, the inhibitor does not affect the amounts of total cofil. 50μM T56-LIMKi causes a statistically significant reduction in the number of cells exhibiting stress fibers [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
T56-LIMKi can induce inhibition of cofilin phosphorylation and Panc-1 tumor shrinkage in vivo . Mice treated with T56-LIMKi (60 mg/kg) shows a significant decrease in tumor volume compared to control [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 100 mg/mL ( 256.85 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.5685 mL | 12.8426 mL | 25.6852 mL |
| 5 mM | 0.5137 mL | 2.5685 mL | 5.1370 mL |
| 10 mM | 0.2569 mL | 1.2843 mL | 2.5685 mL |
-
1.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (6.42 mM); Clear solution
References
- [1] Rak R, et al. Novel LIMK2 Inhibitor Blocks Panc-1 Tumor Growth in a mouse xenograft model. Oncoscience. 2014 Jan 1;1(1):39-48. eCollection 2014.
- [2] Mashiach-Farkash E, et al. Computer-based identification of a novel LIMK1/2 inhibitor that synergizes with salirasib to destabilize the actin cytoskeleton. Oncotarget. 2012 Jun;3(6):629-39.