[CAS NO. 924811-53-0] BT18
PRODUCTS SPECIFICATIONS [924811-53-0]
DESCRIPTION [924811-53-0]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 637.64 |
| Molecular Formula | C30H31F4N3O6S |
| SMILES | O=C(N1CCN(C2=CC(S(=O)(N3CC4=C(C=C(OC)C(OC)=C4)CC3)=O)=CC=C2OC)CC1)C5=CC=C(F)C=C5C(F)(F)F |
Summary
BT18 is a molecule mimic with function similar to glial cell line-derived neurotrophic factor (GDNF) [1] . BT18 shows an effect on GDNF family receptor GFRα1 and RET receptor tyrosine kinase RetA function [2] .
In Vitro
BT18 (50 μM; 1-90 minutes) activates AKT and ERK1/2 in time-dependently in MG87RET cell lines
[1]
.
BT18 (1-50 μM; 90 minutes) activates AKT in a dose-dependent manner in MG87RET cell lines
[1]
.
BT18 (5-50 μM; 15 mimutes) appears to phosphorylate RET in concentration of 50 µM both in GFP-transfected and in GFRD1-transfected cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | MG87RET cell lines |
| Concentration: | 50 μM |
| Incubation Time: | 1, 5, 15, 45, 90 minutes |
| Result: | Activated AKT and ERK1/2 in time-dependently. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 100 mg/mL ( 156.83 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.5683 mL | 7.8414 mL | 15.6828 mL |
| 5 mM | 0.3137 mL | 1.5683 mL | 3.1366 mL |
| 10 mM | 0.1568 mL | 0.7841 mL | 1.5683 mL |
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1.
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2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (3.92 mM); Clear solution
References