[CAS NO. 929895-45-4] Phen-DC3Trifluoromethanesulfonate
PRODUCTS SPECIFICATIONS [929895-45-4]
DESCRIPTION [929895-45-4]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 848.75 |
| Molecular Formula | C36H26F6N6O8S2 |
| SMILES | [O-]S(=O)(C(F)(F)F)=O.C[N+]1=C2C=CC=CC2=CC(NC(C3=CC=C4C=CC5=CC=C(C(NC6=C[N+](C)=C7C=CC=CC7=C6)=O)N=C5C4=N3)=O)=C1.[O-]S(=O)(C(F)(F)F)=O |
3 Publications Citing Use of MCE
Summary
Phen-DC3 Trifluoromethanesulfonate is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC 50 s of 65±6 and 50±10 nM, respectively.
IC50 & Target
IC50: 65±6 nM (G4 substrate, FANCJ helicase), 50±10 nM (G4 substrate, DinG helicases) [1]
In Vitro
In WT cells, a CEB1-WT array is rather stable but undergoes frequent rearrangements upon addition of 10 μM Phen-DC3 Trifluoromethanesulfonate (Phen-DC3). It is found that the c-Myc allele exhibits significant destabilization upon Phen-DC3 Trifluoromethanesulfonate treatment and PIF1 deletion. The CEB25-L111(T) array is stable in WT cells, it becomes unstable upon addition of Phen-DC3 Trifluoromethanesulfonate or deletion of PIF1. It is also highly destabilized in the presence of Phen-DC3 Trifluoromethanesulfonate or in the absence of PIF1. The CEB1-loop CEB25 allele remaines fully stable in both PIF1-treated and WT cells [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : ≥ 34 mg/mL ( 40.06 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.1782 mL | 5.8910 mL | 11.7820 mL |
| 5 mM | 0.2356 mL | 1.1782 mL | 2.3564 mL |
| 10 mM | 0.1178 mL | 0.5891 mL | 1.1782 mL |
References
- [1] Sanjay Kumar Bharti, et al. Specialization among Iron-Sulfur Cluster Helicases to Resolve G-quadruplex DNA Structures That Threaten Genomic Stability. J Biol Chem. 2013 Sep 27; 288(39): 28217–28229.
- [2] Aurèle Piazza, et al. Short loop length and high thermal stability determine genomic instability induced by G-quadruplex-forming minisatellites. EMBO J. 2015 Jun 12; 34(12): 1718–1734.