[CAS NO. 99420-15-2] BTZO-1
PRODUCTS SPECIFICATIONS [99420-15-2]
DESCRIPTION [99420-15-2]
Overview
| MDL | MFCD19690951 |
|---|---|
| Molecular Weight | 240.28 |
| Molecular Formula | C13H8N2OS |
| SMILES | O=C1N=C(C2=NC=CC=C2)SC3=CC=CC=C13 |
Summary
BTZO-1 binds to Macrophage migration inhibitory factor (MIF) with a K d value of 68.6 nM, and its binding requires the N-terminal Pro1. BTZO-1 can activate antioxidant response element ( ARE )-mediated gene expression and suppress oxidative stress-induced cardiomyocyte apoptosis in vitro [1] .
IC50 & Target
Kd:68.6 nM (Macrophage migration inhibitory factor) [1]
In Vitro
BTZO-1 (0-330 nM; 24 hours) dose-dependently suppressed serum deprivation-induced cardiomyocyte death; the minimum effective concentration (MEC1.5), the concentration of a sample necessary to give a 50% increase in cell viability, is 16 nM
[1]
.
BTZO-1 (0.37 μM; 21 hours) upregulates mRNA protein levels of GST Ya and HO-1 in cardiomyocytes
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | Primary Neonatal Rat Cardiomyocytes |
| Concentration: | 0-330 nM |
| Incubation Time: | 24 hours |
| Result: | Suppressed serum deprivation or DOX-induced cell death. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 8.33 mg/mL ( 34.67 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 4.1618 mL | 20.8091 mL | 41.6181 mL |
| 5 mM | 0.8324 mL | 4.1618 mL | 8.3236 mL |
| 10 mM | 0.4162 mL | 2.0809 mL | 4.1618 mL |